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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Fig. 1

a SDS-PAGE analysis showing expression of recombinant OVGP1. Lane 1: Uninduced, lane 2: Molecular weight marker, lane 3: Induced, lane 4: Pellet, lane 5: Soluble fraction; (b) SDS-PAGE analysis of His-tag affinity purified recombinant OVGP1 (indicated by arrow). Lane 1: Molecular weight marker, lane 2–5: Eluted fractions for partially purified His- tagged recombinant OVGP1; (c) Anion- exchange purification of recombinant OVGP1 (indicated by arrow). Lane 1: Unbound fraction, lane 2: Molecular weight marker, lane 3–9: Eluted fractions for partially purified His- tagged recombinant OVGP1; and (d) Gel filtration purification representing single band (indicated by arrow) for purified His- tagged recombinant OVGP1, lane 1: Unstained molecular weight marker, lane 2: purified His- tagged recombinant OVGP1; (e) Western blot analysis of purified His-tagged recombinant OVGP1. Lane 1: Prestained molecular weight marker, lane 2: purified His- tagged recombinant OVGP1

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