Table 2 Primers of target genes for RT-PCR
Genes | GenBank accession | Primers sequences(5′ to 3′) | Product size, bp | Annealing temperature, °C |
|---|---|---|---|---|
β-actin | XM_003124280.3 | CCTGCGGCATCCACGAAAC | 123 | 63 |
TGTCGGCGATGCCTGGGTA | ||||
AMPKα2 | AY159788.1 | GGTCTGGTTCCTCAACACCTCA | 90 | 63 |
GGCTCTCCGCAGTGACAGAAT | ||||
PPARγ | NM_214379 | GTGGAGACCGCCCAGGTTTG | 108 | 64 |
GGGAGGACTCTGGGTGGTTCA | ||||
LPL | NM_214286.1 | CCCTATACAAGAGGGAACCGGAT | 138 | 63 |
CCGCCATCCAGTCGATAAACGT | ||||
CPT1 | NM_001007191.1 | GGACGAGGAGTCTCACCACTATGAC | 128 | 63 |
TCTTGAACGCGATGAGGGTGA | ||||
FATP1 | NM_001083931.1 | CCCTCTGCGTCGCTTTGATG | 151 | 63 |
GCTGCGGTCCCGGAAATACA | ||||
FAT/CD36 | NM_001044622.1 | CTGGTGCTGTCATTGGAGCAGT | 160 | 63 |
CTGTCTGTAAACTTCCGTGCCTGTT | ||||
FABP3 | NM_001099931.1 | CCAACATGACCAAGCCTACCACA | 176 | 63 |
ACAAGTTTGCCTCCATCCAGTGT | ||||
PPARα | NM_001044526.1 | GGCTTACGGCAATGGCTTCA | ||
CGGTCTCCGCACCAAATGA | 168 | 64 |