Fig. 3

Oocytes from obese mice exhibit higher [Ca²⁺]_m and increased apoptosis. a [Ca²⁺]_m levels in oocytes from control and obese mice. Scale bars, 50 μm. b Quantitative analysis of [Ca²⁺]_m. N = 30 per group across three replicates. c Western blotting for activated caspase-3, cytochrome C, P-MAPK-3/1, and MAPK-3/1. d Statistical analysis of activated caspase-3 and cytochrome C levels. e Statistical analysis of P-MAPK-3/1 and MAPK-3/1 levels. N = 360 per group across three replicates. f ROS (green) and GSH (blue) levels in control and obese mice. Scale bars, 100 μm. g Quantitative analysis of ROS and GSH levels. N = 30 per group across three replicates. Graphs show means ± SEM. * P < 0.05, ** P < 0.01, CD vs. HFD; t-test