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Table 7 Effects of methionine on superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reducase (GR) activities and glutathione (GSH) contents in the hepatopancreas and intestine of sub-adult grass carp 1

From: The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella)

Met, g/kg diet

2.21

4.24

6.22

8.25

10.24

12.26

Hepatopancreas

 SOD, U/mg protein

85.06 ± 6.35a

96.33 ± 8.17b

109.44 ± 4.24c

97.08 ± 4.47b

95.10 ± 6.64b

94.63 ± 5.13b

 CAT, U/mg protein

29.91 ± 2.75bc

28.57 ± 2.40b

22.03 ± 2.23a

27.32 ± 0.97b

32.08 ± 3.44c

36.53 ± 3.03d

 GST, U/mg protein

57.57 ± 2.39d

28.52 ± 2.45b

23.22 ± 2.31a

20.79 ± 1.50a

26.27 ± 1.72b

44.93 ± 2.03c

 GPx, U/mg protein

1123.5 ± 67.1

1126.8 ± 53.8

1152.7 ± 62.8

1168.2 ± 39.4

1168.0 ± 57.8

1149.5 ± 56.6

 GR, U/g protein

3.93 ± 0.35a

7.73 ± 0.43c

8.21 ± 0.72c

4.50 ± 0.41ab

4.68 ± 0.38b

4.04 ± 0.32ab

 GSH, mg/g protein

7.11 ± 0.40ab

9.11 ± 0.53c

8.77 ± 0.61c

7.12 ± 0.32ab

7.28 ± 0.32b

6.60 ± 0.26a

Intestine

 SOD, U/mg protein

21.32 ± 1.65a

25.52 ± 1.27b

29.83 ± 2.41c

26.13 ± 2.01b

25.65 ± 1.20b

25.75 ± 3.03b

 CAT, U/mg protein

12.80 ± 1.35

14.02 ± 0.93

13.27 ± 0.37

13.48 ± 1.03

12.62 ± 1.2

13.28 ± 1.18

 GST, U/mg protein

14.80 ± 0.85b

14.88 ± 1.30b

12.84 ± 1.26a

14.08 ± 1.20ab

13.10 ± 1.28a

13.94 ± 1.37ab

 GPx, U/mg protein

85.41 ± 8.12a

107.28 ± 11.02b

101.91 ± 10.68b

89.68 ± 9.60a

80.54 ± 6.69a

80.98 ± 5.46a

 GR, U/g protein

15.47 ± 1.36a

23.81 ± 2.29b

40.44 ± 3.90c

26.51 ± 2.28b

25.67 ± 2.39b

26.27 ± 1.62b

 GSH, mg/g protein

0.63 ± 0.07a

2.82 ± 0.16e

3.70 ± 0.24f

2.51 ± 0.11d

2.31 ± 0.10c

2.01 ± 0.14b

Regressions

 Y CAT in Hepatopancreas = 0.327X2–4.037X + 37.644

R 2 = 0.846

P = 0.061

 Y GST in Hepatopancreas = 1.246X2–19.063X + 91.601

R 2 = 0.969

P = 0.006

  1. a-fMeans in the same row with different superscript letters differ significantly (P < 0.05)
  2. 1Values are mean ± SD (n = 6)