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Table 1 Composition of the experimental diets

From: The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella)

 

Diets, g/kg

Ingredients

Diet 1

Diet 2

Diet 3

Diet 4

Diet 5

Diet 6

Anchovy fish meala

68.00

68.00

68.00

68.00

68.00

68.00

Caseina

30.00

30.00

30.00

30.00

30.00

30.00

Gelatina

39.90

39.90

39.90

39.90

39.90

39.90

Amino acid mixb

150.22

150.22

150.22

150.22

150.22

150.22

α-Starch

280.00

280.00

280.00

280.00

280.00

280.00

Anchovy fish oilc

22.80

22.80

22.80

22.80

22.80

22.80

Soybean oil

18.90

18.90

18.90

18.90

18.90

18.90

Vitamin premixd

10.00

10.00

10.00

10.00

10.00

10.00

Mineral premixe

20.00

20.00

20.00

20.00

20.00

20.00

Ca(H2PO4)2

22.90

22.90

22.90

22.90

22.90

22.90

Choline chloride (500 g/kg)

6.00

6.00

6.00

6.00

6.00

6.00

α-Cellulose

150.00

150.00

150.00

150.00

150.00

150.00

Ethoxyquin (300 g/kg)

0.50

0.50

0.50

0.50

0.50

0.50

DL-Methionine

0.00

2.02

4.04

6.06

8.08

10.10

Glycine

49.09

48.08

47.07

46.06

45.05

44.04

Corn starch

131.69

130.68

129.67

128.66

127.65

126.64

  1. aFish meal was purchased from Pesquera Lota Protein Ltd. (Lota, Chile), casein was purchased from Hulunbeier Sanyuan Milk Co., Ltd. (Inner Mongolia, China), gelatin was purchased from Rousselot Gelatin Co., Ltd. (Guangdong, China)
  2. bAmino acid mix (g/kg): arginine (100%), 11.80 g; histidine (73.3%), 7.23 g; isoleucine (99%), 11.82 g; leucine (99%), 18.99 g; lysine (78.8%), 15.99 g; cystine (99%), 0.81 g; phenylalanine (99%), 12.53 g; tyrosine (99%), 10.00 g; threonine (98%), 11.22 g; tryptophan (98%), 3.27 g; valine (99%), 14.24 g; glutamic acid (99%), 32.32 g
  3. cFish oil was purchased form CIA. Pesquera Camanchaca S.A. (Santiago, Chile)
  4. dPer kilogram of vitamin premix (g/kg): retinyl acetate (500,000 IU/g), 0.800 g; cholecalciferol (500,000 IU/g), 0.480 g; DL-α-tocopherol acetate (500 g/kg), 20.000 g; menadione (23%), 0.220 g; thiamine hydrochloride (98%), 0.120 g; riboflavin (80%), 0.990 g; pyridoxine hydrochloride (98%), 0.620 g; cyanocobalamin (1%), 0.100 g; niacin (99%), 2.58 g; D-biotin (2%), 5.000 g; meso-inositol (99%), 52.330 g; folic acid (96%), 0.520 g; ascorhyl acetate (93%), 7.160 g; calcium-D-pantothenate (90%), 2.780 g. All ingredients were diluted with corn starch to 1 kg
  5. ePer kilogram of mineral premix (g/kg): FeSO4·H2O, 25.00 g; CuSO4·5H2O, 0.60 g; ZnSO4·H2O, 4.35 g; MnSO4·H2O, 2.04 g; KI, 1.10 g; NaSeO3, 2.50 g; MgSO4·H2O, 230.67 g. All ingredients were diluted with corn starch to 1 kg