Long-term effects of early antibiotic intervention on blood parameters, apparent nutrient digestibility, and fecal microbial fermentation profile in pigs with different dietary protein levels

Table 2 Primers used for quantification in this study

Target	Primer sequence 5′→3′	Reference
Total bacteria	Forward: CGGTGAATACGTTCYCGG Reverse: GGWTACCTTGTTACGACTT	[38]
Firmicutes	Forward: GGAGYATGTGGTTTAATTCGAAGCA	[39]
Firmicutes	Reverse: AGCTGACGACAACCATGCAC	[39]
Bacteroidetes	Forward: GGARCATGTGGTTTAATTCGATGAT	[39]
Bacteroidetes	Reverse: AGCTGACGACAACCATGCAG	[39]
Clostridium IV	Forward: GCACAAGCAGTGGAGT	[40]
Clostridium IV	Reverse: CTTCCTCCGTTTTGTCAA	[40]
Clostridium XIVa	Forward: CGGTACCTGACTAAGAAGC	[41]
Clostridium XIVa	Reverse: AGTTTYATTCTTGCGAACG	[41]
E.coli	Forward: CATGCCGCGTGTATGAAGAA	[42]
E.coli	Reverse: CGGGTAACGTCAATGAGCAAA	[42]
Lactobacillus	Forward: AGCAGTAGGGAATCTTCCA	[43]
Lactobacillus	Reverse: ATTCCACCGCTACACATG	[43]

ISSN: 2049-1891