Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

Table 2 Log₂ back-transformed LSM of gene transcription for treatment (TRT) and incubation time (T), SEM and P values for TRT and T

	LSM TRT^d				LSM T		SEM		P-value
Gene	Control	LPS	LTA	L + L	3 h	6 h	TRT	T	TRT	T
Cytokines
CCL2	0.49^c	1.61^a	0.54^c	0.80^b	0.72	0.80	0.08	0.06	<0.0001	0.0637
CCL5	1.64^b	1.58^b	1.65^b	1.91^a	1.60^z	1.78^y	0.06	0.04	0.0022	0.0034
CXCL6	0.43^c	1.58^a	0.40^c	0.96^b	0.78^y	0.66^z	0.08	0.06	<0.0001	0.0093
CXCL8	0.51^c	1.64^a	0.43^c	0.97^b	0.97^y	0.61^z	0.12	0.09	<0.0001	<0.0001
IL6	1.26^b	2.65^a	1.27^b	1.90^a	1.66	1.71	0.22	0.18	0.0004	0.8208
Regulatory genes
IFIT3	1.01^c	1.13^b	1.20^ab	1.24^a	0.95^z	1.37^y	0.04	0.03	<0.0001	<0.0001
IRF3	1.05^b	1.09	1.13	1.19^a	1.01^z	1.22^y	0.05	0.04	0.0818	<0.0001
MYD88	1.73^b	2.05^a	1.81^b	2.11^a	1.81^z	2.03^y	0.03	0.03	<0.0001	<0.0001
NFKB1	1.13^c	1.49^a	1.34^b	1.51^a	1.10^z	1.68^y	0.05	0.04	<0.0001	<0.0001
PTGS2	1.05^b	1.30^a	1.32^a	1.29^a	1.03^z	1.48^y	0.05	0.04	<0.0001	<0.0001
TLR2	10.39	11.38	10.11	11.24	10.58	10.96	0.09	0.07	0.3747	0.5420
TLR4	1.04^c	1.49^a	1.04^c	1.22^b	1.18	1.19	0.08	0.06	<0.0001	0.8645
TOLLIP	0.96^b	0.96^b	1.04^a	0.95^b	0.98	0.97	0.02	0.01	<0.0001	0.3915

^a-cDifferent letters represent significant differences between treatments (P < 0.05)
The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
^dTreatments: Control = incubation without toxins; LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins
^y-zDifferent letters represent significant differences between time points (P < 0.05). The letter y indicates higher transcript levels than z