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Table 2 Log2 back-transformed LSM of gene transcription for treatment (TRT) and incubation time (T), SEM and P values for TRT and T

From: Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

 

LSM TRTd

LSM T

SEM

P-value

Gene

Control

LPS

LTA

L + L

3 h

6 h

TRT

T

TRT

T

Cytokines

  CCL2

0.49c

1.61a

0.54c

0.80b

0.72

0.80

0.08

0.06

<0.0001

0.0637

  CCL5

1.64b

1.58b

1.65b

1.91a

1.60z

1.78y

0.06

0.04

0.0022

0.0034

  CXCL6

0.43c

1.58a

0.40c

0.96b

0.78y

0.66z

0.08

0.06

<0.0001

0.0093

  CXCL8

0.51c

1.64a

0.43c

0.97b

0.97y

0.61z

0.12

0.09

<0.0001

<0.0001

  IL6

1.26b

2.65a

1.27b

1.90a

1.66

1.71

0.22

0.18

0.0004

0.8208

Regulatory genes

  IFIT3

1.01c

1.13b

1.20ab

1.24a

0.95z

1.37y

0.04

0.03

<0.0001

<0.0001

  IRF3

1.05b

1.09

1.13

1.19a

1.01z

1.22y

0.05

0.04

0.0818

<0.0001

  MYD88

1.73b

2.05a

1.81b

2.11a

1.81z

2.03y

0.03

0.03

<0.0001

<0.0001

  NFKB1

1.13c

1.49a

1.34b

1.51a

1.10z

1.68y

0.05

0.04

<0.0001

<0.0001

  PTGS2

1.05b

1.30a

1.32a

1.29a

1.03z

1.48y

0.05

0.04

<0.0001

<0.0001

  TLR2

10.39

11.38

10.11

11.24

10.58

10.96

0.09

0.07

0.3747

0.5420

  TLR4

1.04c

1.49a

1.04c

1.22b

1.18

1.19

0.08

0.06

<0.0001

0.8645

  TOLLIP

0.96b

0.96b

1.04a

0.95b

0.98

0.97

0.02

0.01

<0.0001

0.3915

  1. a-cDifferent letters represent significant differences between treatments (P < 0.05)
  2. The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
  3. dTreatments: Control = incubation without toxins; LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins
  4. y-zDifferent letters represent significant differences between time points (P < 0.05). The letter y indicates higher transcript levels than z