|
LSM TRTd
|
LSM T
|
SEM
|
P-value
|
---|
Gene
|
Control
|
LPS
|
LTA
|
L + L
|
3 h
|
6 h
|
TRT
|
T
|
TRT
|
T
|
---|
Cytokines
|
CCL2
|
0.49c
|
1.61a
|
0.54c
|
0.80b
|
0.72
|
0.80
|
0.08
|
0.06
|
<0.0001
|
0.0637
|
CCL5
|
1.64b
|
1.58b
|
1.65b
|
1.91a
|
1.60z
|
1.78y
|
0.06
|
0.04
|
0.0022
|
0.0034
|
CXCL6
|
0.43c
|
1.58a
|
0.40c
|
0.96b
|
0.78y
|
0.66z
|
0.08
|
0.06
|
<0.0001
|
0.0093
|
CXCL8
|
0.51c
|
1.64a
|
0.43c
|
0.97b
|
0.97y
|
0.61z
|
0.12
|
0.09
|
<0.0001
|
<0.0001
|
IL6
|
1.26b
|
2.65a
|
1.27b
|
1.90a
|
1.66
|
1.71
|
0.22
|
0.18
|
0.0004
|
0.8208
|
Regulatory genes
|
IFIT3
|
1.01c
|
1.13b
|
1.20ab
|
1.24a
|
0.95z
|
1.37y
|
0.04
|
0.03
|
<0.0001
|
<0.0001
|
IRF3
|
1.05b
|
1.09
|
1.13
|
1.19a
|
1.01z
|
1.22y
|
0.05
|
0.04
|
0.0818
|
<0.0001
|
MYD88
|
1.73b
|
2.05a
|
1.81b
|
2.11a
|
1.81z
|
2.03y
|
0.03
|
0.03
|
<0.0001
|
<0.0001
|
NFKB1
|
1.13c
|
1.49a
|
1.34b
|
1.51a
|
1.10z
|
1.68y
|
0.05
|
0.04
|
<0.0001
|
<0.0001
|
PTGS2
|
1.05b
|
1.30a
|
1.32a
|
1.29a
|
1.03z
|
1.48y
|
0.05
|
0.04
|
<0.0001
|
<0.0001
|
TLR2
|
10.39
|
11.38
|
10.11
|
11.24
|
10.58
|
10.96
|
0.09
|
0.07
|
0.3747
|
0.5420
|
TLR4
|
1.04c
|
1.49a
|
1.04c
|
1.22b
|
1.18
|
1.19
|
0.08
|
0.06
|
<0.0001
|
0.8645
|
TOLLIP
|
0.96b
|
0.96b
|
1.04a
|
0.95b
|
0.98
|
0.97
|
0.02
|
0.01
|
<0.0001
|
0.3915
|
-
a-cDifferent letters represent significant differences between treatments (P < 0.05)
- The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
-
dTreatments: Control = incubation without toxins; LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins
-
y-zDifferent letters represent significant differences between time points (P < 0.05). The letter y indicates higher transcript levels than z