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Table 2 Log2 back-transformed LSM of gene transcription for treatment (TRT) and incubation time (T), SEM and P values for TRT and T

From: Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

  LSM TRTd LSM T SEM P-value
Gene Control LPS LTA L + L 3 h 6 h TRT T TRT T
Cytokines
CCL2 0.49c 1.61a 0.54c 0.80b 0.72 0.80 0.08 0.06 <0.0001 0.0637
CCL5 1.64b 1.58b 1.65b 1.91a 1.60z 1.78y 0.06 0.04 0.0022 0.0034
CXCL6 0.43c 1.58a 0.40c 0.96b 0.78y 0.66z 0.08 0.06 <0.0001 0.0093
CXCL8 0.51c 1.64a 0.43c 0.97b 0.97y 0.61z 0.12 0.09 <0.0001 <0.0001
IL6 1.26b 2.65a 1.27b 1.90a 1.66 1.71 0.22 0.18 0.0004 0.8208
Regulatory genes
IFIT3 1.01c 1.13b 1.20ab 1.24a 0.95z 1.37y 0.04 0.03 <0.0001 <0.0001
IRF3 1.05b 1.09 1.13 1.19a 1.01z 1.22y 0.05 0.04 0.0818 <0.0001
MYD88 1.73b 2.05a 1.81b 2.11a 1.81z 2.03y 0.03 0.03 <0.0001 <0.0001
NFKB1 1.13c 1.49a 1.34b 1.51a 1.10z 1.68y 0.05 0.04 <0.0001 <0.0001
PTGS2 1.05b 1.30a 1.32a 1.29a 1.03z 1.48y 0.05 0.04 <0.0001 <0.0001
TLR2 10.39 11.38 10.11 11.24 10.58 10.96 0.09 0.07 0.3747 0.5420
TLR4 1.04c 1.49a 1.04c 1.22b 1.18 1.19 0.08 0.06 <0.0001 0.8645
TOLLIP 0.96b 0.96b 1.04a 0.95b 0.98 0.97 0.02 0.01 <0.0001 0.3915
  1. a-cDifferent letters represent significant differences between treatments (P < 0.05)
  2. The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
  3. dTreatments: Control = incubation without toxins; LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins
  4. y-zDifferent letters represent significant differences between time points (P < 0.05). The letter y indicates higher transcript levels than z