Table 1 List of primer sequences used for cloning of the CVH promoter
Primer sets | Primer sequence (5′ → 3′) |
|---|---|
CVH −1,575 bp_F | GACACAGCTTTCCCACGTGAG |
CVH −1,231 bp_F | TGGCCACGTGCTATCATATTAGT |
CVH −625 bp_F | CTCTGATCATGCCTGCAGCC |
CVH −316 bp_F | CAGGACAGGCCTAGGGACAGA |
CVH −227 bp_F | AGCATAAACAGGGAAAGCGC |
CVH −135 bp_F | GCGCCACCTTCTCACCCC |
CVH +25 bp_F | GCTATTTGGAGCGGAGAGTGAAA |
CVH promoter_R | AGCGAATGCCAGCAGCC |
CVH −135/+222_R | CGCCCTGACGCCACCAT |
CVH −135/+162_R | AGCACGCACTGCCCTTGC |
eGFP poly A _F | ACTAGTCCGCGGATGGTGAGCAAG |
eGFP poly A_R | CATATGGACGTCTCCCCAGCATGCC |