Fig. 5

Relative gene expression analysis after knockdown of predicted TFs in cultured PGCs. a–f Confirmation of siRNA knockdown efficiency in vitro in chicken PGCs for the predicted TFs as determined by qRT-PCR. g–l Relative expression analysis of germ cell-related RNA binding proteins (CVH, cDAZL, CIWI, and CDH) in cultured PGCs after treatment of each siRNA. ACTB (beta-actin) was used as a control for silencing the specificity of the knockdown probes. qPCR was conducted in triplicate, with data being normalized to the expression of GAPDH as a control. Significant differences between control and treatment groups are indicated as ***P < 0.001, **P < 0.01, and *P < 0.05. Error bars indicate the standard error of triplicate analyses