Fig. 4

Quantitative expression analysis of predicted TFs in various cell types. By quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis, predicted TFs were analyzed with the prepared PGCs, Stage X blastoderm, chicken embryonic fibroblasts, DF-1, and GSCs. Error bars indicate the standard deviation of triplicate analysis. Significant differences are indicated as ***P < 0.001 and **P < 0.01