Fig. 3

Location of predicted transcription factor (TF) binding sites on the CVH promoter. a A flowchart of the process of selection of TFs having putative binding sites in the 591-bp fragment (−316/+275) of the CVH promoter. The input is sets of TFs predicted by several prediction software programs (MatInspector, PROMO, and TFBIND). TFs with a similarity score below 0.8 were then removed. The putative TFs were extracted depending on significant expression in chicken PGCs, from the transcriptome data. b Nucleotide sequences of the 5′ flanking region of the 591-bp fragment (−316/+275) of the CVH promoter. Selected TFs were marked on the sequences of the CVH promoter region. c Schematic diagrams of the locations of these predicted factors in the promoter region. +1 indicates the transcriptional initiation site and boxed ATG indicates the translational start site. The bold sequences show predicted binding sites of the TFs. Underline indicates the intron region of the CVH gene. GSCs, gonadal stromal cells