Short-term effect of supplemental yeast extract without or with feed enzymes on growth performance, immune status and gut structure of weaned pigs challenged with Escherichia coli lipopolysaccharide

Table 2 Primers used for qPCR analysis of immune cytokines

Gene^a	GenBank reference no.	Amplicon size, bp	T_m ^b, °C	Primer sequence (5′→3′)
IFN-γ	NM_213948.1	231	60	F: GGCCATTCAAAGGAGCATGG R: GCTCTCTGGCCTTGGAACAT
IL-1β	NM_214055.1	131	60	F: GCCCATCATCCTTGAAACGTG R: GGAGAGCCTTCAGCATGTGT
IL-6	NM_214399.1	149	60	F: TAAGGGAAATGTCGAGGCCG R: TCCACTCGTTCTGTGACTGC
IL-10	NM_214041.1	179	60	F: TCCGACTCAACGAAGAAGGC R: AACTCTTCACTGGGCCGAAG
TNF-α	NM_214022.1	120	60	F: ATTCAGGGATGTGTGGCCTG R: CCAGATGTCCCAGGTTGCAT
β-actin	XM_005670976.1	179	60	F: CGAGGCTCAGAGCAAGAGAG R: GGTTGGCCTTAGGGTTCAGG

ISSN: 2049-1891