Table 1 Primers used in this study
Gene | Primer sequence(5’-3’) | T m(°C) | Size,bp |
|---|---|---|---|
β-actin | Forward: GTGGACATCAGGAAGGACCTCTA Reverse: ATGATCTTGATCTTCATGGTGCT | 60.0 | 131 |
HSP70 | Forward: TTCGACGTGTCCATCCTGACG Reverse: TCACCGCCCGCTTGTTCTGG | 60.0 | 169 |
Sphk1 | Forward: CCAGGTGCACCCAAACTACT Reverse: GAGGCTTCTGGCTGAGTGAG | 60.0 | 92 |
SOD1 | Forward: TCCATGTCCATCAGTTTGGA Reverse: AGTCACATTGGCCCAGGTCTC | 60.0 | 131 |
CYP11A | Forward:TGGTGACAATGGCTGGATTAACCT Reverse: GACGAAGTCCTGAGACACTGTGT | 60.0 | 376 |
CYP19 | Forward: GTCGTGGACTTCGTCATG Reverse: AGTGTGACCAAGATGACCTT | 60.0 | 257 |
SIRT1 | Forward: TTGATCTTCTCATTGTTATTGGGTC Reverse: ACTTGGAATTAGTGCTACTGGTCTTA | 60.0 | 62 |
Akt2 | Forward: AAAGTCATCCTGGTGCG Reverse: GGGTGCCTGGTGTTCTG | 60.0 | 136 |