Table 1 Target region, sequence, and amplicon size of the primers used for SNP identification
From: Gene expression of vascular endothelial growth factor A and hypoxic adaptation in Tibetan pig
Primer | Target region | Forward primer sequence (5ʹ to 3ʹ) | Reverse primer sequence (5ʹ to 3ʹ) | Amplicon size, bp |
|---|---|---|---|---|
5ʹ- FR1 | −1902/−2693 | AGTGACTGGCTCCTGTTCTC | CCTGGGTAGAAGTATTTGGC | 791 |
5ʹ- FR2 | −2193/−1902 | CGTTCCTTAGTGCTGGTGAG | AAAGTGAGGTTATGTGCGGC | 843 |
5ʹ- FR3 | −1546/−631 | GTGTGTCTGGGTGTGTGTGG | TCCCTCTCGTTTCTTGCTTGC | 915 |
5ʹ- FR4 | −654/+53 | GGGCAAGCAAGAAACGAGA | AGGTAGAGCAGCAAGGCAA | 707 |
VEGFA-P1 | Exon1 | GAGGAGGAAGAAGAGAAGGAAG | CATGTACGAGGATAGAGGGGAA | 472 |
VEGFA-P2 | Exon2 | CCATTCTTCCCTCTTTGTTTTGTC | TTTGTTTTCCCAGTCTGTGCTCA | 367 |
VEGFA-P3 | Exon3 | GGCCGGCCCCCTCTACAG | AACGGGCTTTTTAAACTCTCCACA | 630 |
VEGFA-P4 | Exon4-5 | CCTGGTCTGTGGAGAGTTTA | AGTGGGTAGAGAAAGAGAAA | 872 |
VEGFA-P5 | Exon6 | CTGCCGCTCTCTCTTGTCTTCTGC | AGCCACGCCTGCCACCTG | 564 |
VEGFA-P6 | Exon7 | CGTAGGGACTCTTCTTTGGT | CTCGGCTTGTCACATCTGC | 313 |