Fig. 3

Relative mRNA abundance for cytokines in primary adipose cultures in response to ORM1 treatment. Primary cultures were derived from neonatal porcine subcutaneous adipose tissue according to procedures described in the Methods section. Cultures containing differentiated adipocytes and stromal-vascular cells were incubated with 0, 100, 1000 or 5000 ng porcine ORM1/mL medium for 24 h. Relative tumor necrosis factor α (TNF, a), interleukin 6 (IL6, b), macrophage migration inhibitor factor (MIF, c), monocyte chemotactic protein (d) and adiponectin (e) mRNA abundance were measured by real time PCR as described in the methodology. The relative expression of the genes of interest, standardized against the amount of cyclophilin mRNA in each cell culture was calculated using the ∆∆CT method. Data are expressed relative to values determined for incubations without ORM1, setting these values to 1. ^abc Means not sharing a common superscript letter are different (n = 5; P < 0.05)