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Table 5 C18 fatty acids (FA) composition and biohydrogenation of C18:2 n-6 and C18:3 n-3 after 6 h in vitro incubation

From: Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria

Fatty acid, % of total FA Sucrose level SEM1 P-value2
  0 3 % 6 % 9 % T L Q
C18:0 22.6 23.2 19.3 17.7 1.146 0.252 0.071 0.620
C18:1 trans-4 0.13 0.20 0.17 0.18 0.011 0.150 0.204 0.197
C18:1 trans-5 0.17a 0.11b 0.15a 0.14ab 0.008 0.017 0.256 0.064
C18:1 tans-6/7/8 3.79 3.84 3.59 3.29 0.135 0.475 0.171 0.531
C18:1 trans-9 0.14 0.11 0.20 0.14 0.020 0.567 0.636 0.799
C18:1 trans-10 0.38a 0.34a 0.24b 0.24b 0.021 0.011 0.002 0.554
C18:1 trans-11 8.70b 8.91b 10.1a 10.5a 0.107 0.040 0.021 0.549
C18:1 cis-9 1.48 1.58 1.75 1.62 0.061 0.565 0.303 0.381
C18:1 cis-6 0.26 0.25 0.22 0.20 0.020 0.702 0.259 0.997
C18:1 cis-11 0.16 0.15 0.081 0.16 0.123 0.093 0.491 0.079
C18:2 trans-9,trans-12 0.11 0.21 0.15 0.18 0.020 0.313 0.386 0.417
C18:2 cis-9,trans-13 0.058 0.048 0.061 0.062 0.004 0.699 0.542 0.579
C18:2 trans-8,cis-13 0.63 0.61 0.60 0.69 0.022 0.435 0.346 0.210
C18:2 cis-9,trans-12 0.064 0.058 0.061 0.078 0.004 0.303 0.209 0.168
C18:2 trans-9,cis-12 0.036 0.033 0.045 0.032 0.004 0.709 0.987 0.556
C18:2 trans-11,cis-15 0.14 0.13 0.12 0.13 0.011 0.962 0.922 0.641
C18:2 n-6 11.0b 13.6ab 16.4a 14.5ab 0.707 0.045 0.021 0.072
C18:3 n-3 2.34 3.00 3.11 2.75 0.114 0.063 0.131 0.021
CLA cis-9,trans-11 0.028 0.043 0.026 0.036 0.004 0.588 0.871 0.811
CLA trans-10,cis-12 0.036 0.033 0.032 0.029 0.003 0.894 0.473 0.985
Trans-11/trans-103 19.1c 21.2bc 33.7ab 40.0a 2.959 0.012 0.002 0.609
Biohydrogenation (%)       
C18:2n-6 58.5a 43.8b 36.2b 36.3b 3.151 0.008 0.002 0.081
C18:3n-3 30.9a 25.8ab 17.4c 19.1bc 1.790 0.015 0.046 0.253
  1. Note: Means within the same row with different superscripts differ significantly (P < 0.05)
  2. 1 SEM = standard error of mean
  3. 2 Probability of a significant effect of treatment (T), or linear (L) and quadratic (Q) orthogonal contrasts for dietary sucrose levels
  4. 3 Tans-11/trans-10 = ratio of total trans-11 C18 isomers (C18:1 trans-11 + CLA cis-9,trans-11) to trans-10 isomers (C18:1 trans-10 + CLA trans-10, cis-12)