Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria

Table 5 C18 fatty acids (FA) composition and biohydrogenation of C18:2 n-6 and C18:3 n-3 after 6 h in vitro incubation

Fatty acid, % of total FA	Sucrose level				SEM¹	P-value²
	0	3 %	6 %	9 %	SEM¹	T	L	Q
C18:0	22.6	23.2	19.3	17.7	1.146	0.252	0.071	0.620
C18:1 trans-4	0.13	0.20	0.17	0.18	0.011	0.150	0.204	0.197
C18:1 trans-5	0.17^a	0.11^b	0.15^a	0.14^ab	0.008	0.017	0.256	0.064
C18:1 tans-6/7/8	3.79	3.84	3.59	3.29	0.135	0.475	0.171	0.531
C18:1 trans-9	0.14	0.11	0.20	0.14	0.020	0.567	0.636	0.799
C18:1 trans-10	0.38^a	0.34^a	0.24^b	0.24^b	0.021	0.011	0.002	0.554
C18:1 trans-11	8.70^b	8.91^b	10.1^a	10.5^a	0.107	0.040	0.021	0.549
C18:1 cis-9	1.48	1.58	1.75	1.62	0.061	0.565	0.303	0.381
C18:1 cis-6	0.26	0.25	0.22	0.20	0.020	0.702	0.259	0.997
C18:1 cis-11	0.16	0.15	0.081	0.16	0.123	0.093	0.491	0.079
C18:2 trans-9,trans-12	0.11	0.21	0.15	0.18	0.020	0.313	0.386	0.417
C18:2 cis-9,trans-13	0.058	0.048	0.061	0.062	0.004	0.699	0.542	0.579
C18:2 trans-8,cis-13	0.63	0.61	0.60	0.69	0.022	0.435	0.346	0.210
C18:2 cis-9,trans-12	0.064	0.058	0.061	0.078	0.004	0.303	0.209	0.168
C18:2 trans-9,cis-12	0.036	0.033	0.045	0.032	0.004	0.709	0.987	0.556
C18:2 trans-11,cis-15	0.14	0.13	0.12	0.13	0.011	0.962	0.922	0.641
C18:2 n-6	11.0^b	13.6^ab	16.4^a	14.5^ab	0.707	0.045	0.021	0.072
C18:3 n-3	2.34	3.00	3.11	2.75	0.114	0.063	0.131	0.021
CLA cis-9,trans-11	0.028	0.043	0.026	0.036	0.004	0.588	0.871	0.811
CLA trans-10,cis-12	0.036	0.033	0.032	0.029	0.003	0.894	0.473	0.985
Trans-11/trans-10³	19.1^c	21.2^bc	33.7^ab	40.0^a	2.959	0.012	0.002	0.609
Biohydrogenation (%)
C18:2n-6	58.5^a	43.8^b	36.2^b	36.3^b	3.151	0.008	0.002	0.081
C18:3n-3	30.9^a	25.8^ab	17.4^c	19.1^bc	1.790	0.015	0.046	0.253

Note: Means within the same row with different superscripts differ significantly (P < 0.05)
¹ SEM = standard error of mean
² Probability of a significant effect of treatment (T), or linear (L) and quadratic (Q) orthogonal contrasts for dietary sucrose levels
³ Tans-11/trans-10 = ratio of total trans-11 C18 isomers (C18:1 trans-11 + CLA cis-9,trans-11) to trans-10 isomers (C18:1 trans-10 + CLA trans-10, cis-12)

ISSN: 2049-1891