Fig. 2
From: Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection
Characterization of transgenic buffalo calves derived from cytoplasmic zygote injection. a. Main features of injected pEGFP-N1 plasmid, showing restriction positions (in brackets), PCR primer binding sites (single arrows), Southern probe location (double block arrow) and expected amplicon sizes. p = promoter, pA = polyA site, R = resistance; b, c. Detection of EGFP by PCR (b) and Southern blot (c) in genomic DNA extracted from biopsied ear tissues of transgenic buffalo calves (male, female) and wild-type buffalo ear tissue (WT). Water provided a no template control. d. Ear tissue sections from transgenic calves (male, female) and wild-type control calf (WT) observed by confocal laser scanning microscopy (Brightfield, EGFP and merged images, respectively)