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Table 8 Effects of dietary threonine levels on GSH content and activities of GR, SOD, CAT, GST and GPx in intestine and hepatoancreas of sub-adult grass carpe

From: Growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine

Item Dietary Thr levels, g/kg diet
  3.3 5.9 8.4 10.9 13.1 15.8
Intestine
 GSH, mg/g protein 1.81 ± 0.16a 1.83 ± 0.13a 2.56 ± 0.19b 3.00 ± 0.19c 5.63 ± 0.22d 2.98 ± 0.16c
 GR, U/g protein 23.15 ± 1.22b 24.57 ± 1.95b 17.05 ± 1.36a 19.27 ± 1.78a 27.65 ± 2.82c 33.59 ± 3.11d
 SOD, U/mg protein 19.84 ± 1.71a 23.87 ± 2.33b 23.62 ± 1.13b 23.41 ± 2.19b 24.83 ± 1.44b 23.45 ± 1.84b
 CAT, U/mg protein 11.70 ± 1.03a 16.65 ± 1.48c 16.77 ± 1.31c 22.59 ± 1.94d 18.06 ± 1.26c 14.41 ± 1.46b
 GST, U/mg protein 7.08 ± 0.75a 17.41 ± 1.58b 16.82 ± 1.48b 16.13 ± 1.41b 17.36 ± 1.33b 17.41 ± 1.58b
 GPx, U/mg protein 105.4 ± 9.7b 109.4 ± 6.6b 109.3 ± 4.1b 107.4 ± 9.3b 104.9 ± 5.7b 87.7 ± 8.8a
Hepatopancreas
 GSH, mg/g protein 6.13 ± 0.30b 7.07 ± 0.41c 7.06 ± 0.31c 7.83 ± 0.39d 6.08 ± 0.29b 5.67 ± 0.31a
 GR, U/g protein 4.08 ± 0.31a 13.22 ± 1.02c 7.74 ± 0.80b 7.91 ± 0.59b 7.36 ± 0.73b 7.58 ± 0.75b
 SOD, U/mg protein 83.16 ± 8.65b 100.42 ± 6.19c 80.39 ± 7.06b 84.66 ± 4.00b 78.72 ± 5.17b 66.57 ± 3.08a
 CAT, U/mg protein 55.67 ± 5.95a 59.11 ± 5.39a 79.98 ± 7.11c 69.65 ± 7.34b 57.94 ± 6.91a 56.51 ± 5.61a
 GST, U/mg protein 22.51 ± 2.05b 45.73 ± 2.73d 44.27 ± 3.78d 25.80 ± 2.68c 25.77 ± 2.48c 17.34 ± 1.66a
 GPx, U/mg protein 961.5 ± 79.2a 1,205.9 ± 44.5c 1,041.0 ± 55.3b 1,053.9 ± 57.1b 1,026.2 ± 46.4ab 995.7 ± 47.2ab
  1. GSH: Glutathione; GR: Glutathione reductase; SOD: Superoxide dismutase; CAT: Catalase; GST: Glutathione-S-transferase; GPx: Glutathione peroxidase
  2. a,b,c,dMeans in the same row without a letter in common are significantly different (P< 0.05)
  3. eValues are mean ± SD (n = 6)