Table 2 Analytical method used for metabolic profile
Components | Method of analysis |
|---|---|
Protein metabolism | |
Total protein (TP) | Biuret Test |
Albumin (Alb) | Bromcresol green (BCG) Method |
Urea (UREA) | Urease-Glutamate dehydrogenase (GLDH) Method |
Energy metabolism | |
Glucose (Glu) | Hexokinase (HK)- |
Glucose-6-phosphate dehydrogenase (G6PD) Method | |
Total cholesterol (T-Cho) | Cholesterol oxidase |
Long-chain fatty acid (LCFA) | Enzymatic colorimetric |
Liver function | |
Aspartate aminotransferase (AST) | Malate dehydrogenase (MDH) UV |
Gamma glutamyl transpeptidase (GGT) | Glu-3-CA-4-NA substrate |
Kidney function | |
Creatinine (Crea) | Jaffe’ Method |
Mineral metabolism | |
Calcium (Ca) | O-Cresolphthalein Complexone (OCPC) Method |
Inorganic phosphorous (iP) | Enzymatic UV |
Magnesium (Mg) | Enzymatic UV |