Figure 1
From: Identification and quantitative mRNA analysis of a novel splice variant of GPIHBP1 in dairy cattle
PCR analysis of the GPIHBP1 coding region in samples of mammary gland using three pairs of primers. (A) With primer 1, transcript X2 (603 bp), transcript X3 (289 bp) and transcript X4 (192 bp) were observed as expected (according to the mRNA sequence of the bovine GPIHBP1 gene in NCBI database). M1: DL2000, 2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp. (B) With primer 2, in addition to the expected PCR band (352 bp and 344 bp), a band of 383 bp was also observed in all samples. M2: 100 bp DNA Ladder from 1,500–100 bp. (C) With primer 3, only the expected band (388 bp) was observed in all samples. M3: DL500, 500 bp, 400 bp, 300 bp, 200 bp, 150 bp, 100 bp and 50 bp.