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Table 1 Primer and probe sequences used in our study

From: A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

Name

Sequence 5′→3′

Location (GenBank no.)

Product

dHP-PRRSV-F

GGGTCGGCACCAGTTCC

1555-1571 (FJ495082.2)

73 bp

dHP-PRRSV-R

AATCCAGAGGCTCATCCTGGT

1607-1627 (FJ495082.2)

dHP-PRRSV-pro

FAM-CACCGCGTATAACTGTGACAACAACGC-BHQ1

1574-1600 (FJ495082.2)