A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

Table 1 Primer and probe sequences used in our study

Name	Sequence 5′→3′	Location (GenBank no.)	Product
dHP-PRRSV-F	GGGTCGGCACCAGTTCC	1555-1571 (FJ495082.2)	73 bp
dHP-PRRSV-R	AATCCAGAGGCTCATCCTGGT	1607-1627 (FJ495082.2)	73 bp
dHP-PRRSV-pro	FAM-CACCGCGTATAACTGTGACAACAACGC-BHQ1	1574-1600 (FJ495082.2)

ISSN: 2049-1891