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Table 3 Effect of phenotypic RFI and diet on ruminalmethanogen populations 1

From: Quantitative analysis of ruminal methanogenic microbial populations in beef cattle divergent in phenotypic residual feed intake (RFI) offered contrasting diets

Items RFI Diet3 Significance4
H L SED HF LF SED RFI Diet RxD
Methanogens1 0.07 0.08 0.010 0.07 0.09 0.010 0.71 0.01 0.08
Methanosphaera stadtmanae 2 0.36 0.37 0.069 0.09 0.64 0.069 0.44 <0.0001 0.68
M smithii 2 0.32 0.25 0.057 0.14 0.43 0.057 0.29 <0.0001 0.33
M ruminantium 2 0.18 0.29 0.089 0.04 0.43 0.089 0.65 <0.0001 0.92
  1. 1Methanogens measured as a proportion of total estimated rumen bacterial 16S rDNA, relative quantification = 2-(Ct target-Ct total bacteria).
  2. 2Methanogen spp. measured as a proportion of total estimated rumen methanogen 16S rDNA, relative quantification = 2-(Ct target-Ct total methanogens).
  3. 3Diet = HF = high forage (grass silage), LF = Low forage (maize silage (30):concentrate (70)).
  4. 4Significance values for transformed data. Back transformed means presented for clarity. R = RFI, D = DIET.