Quantitative analysis of ruminal methanogenic microbial populations in beef cattle divergent in phenotypic residual feed intake (RFI) offered contrasting diets

Table 2 PCR primers and probes used in this study for TaqManqRT-PCR analysis

*Primer/Probe*	Sequence (5’→3’)	Efficiency	Product size, bp	Reference
Msmithii F	CCGGGTATCTAATCCGGTTC			Dridi et al., 2009 [19]
Msmithii R	CTCCCAGGGTAGAGGTGAAA	1.98	123	Dridi et al., 2009 [19]
Msmithii probe	CCGTCAGAATCGTTCCAGTCAG			Dridi et al., 2009 [19]
Mruminantium F	CCGGGTATCTAATCCGGTTC			Dridi et al., 2009 [19]
Mruminantium R	CTCCCAGGGTAGAGGTGAAA	2.02	123	Dridi et al., 2009 [19]
Mruminantium probe	CCGTCAGGTTCGTTCCAGTTAG			Current study

ISSN: 2049-1891