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Table 3 FA composition of 3T3-L1 adipocytes overexpressing DGAT2 cultured with FA-DM

From: Effect of diacylglycerol acyltransferase 2 overexpression in 3T3-L1 is associated to an increase in mono-unsaturated fatty acid accumulation

Fatty acids 30 min 12 d
  Control DGAT2 Control DGAT2
Myristic acid (C14:0) 2.70 ± 0.15 2.62 ± 0.49 2.84 ± 0.42 2.42 ± 0.29
Palmitoleic acid (C16:1) 2.67 ± 0.15 3.86 ± 0.33* 2.61 ± 0.17 3.54 ± 0.33*
Palmitic acid (C16:0) 39.11 ± 4.55 33.61 ± 2.104 31.17 ± 1.1003 29.23 ± 1.34
Linoleic acid (C18:2) 5.14 ± 0.38 6.63 ± 1.74 8.44 ± 0.29 11.38 ± 0.41**
Oleic acid (C18:1) 14.18 ± 1.19 12.31 ± 1.07 8.66 ± 0.32 16.47 ± 0.52**
Stearic acid (C18:0) 22.95 ± 2.33 26.45 ± 0.99 26.76 ± 0.42 22.54 ± 0.90**
Arachidonic acid (C20:4) 12.29 ± 1.88 13.56 ± 1.99 15.65 ± 0.46 9.86 ± 1.10**
Arachidic acid (C20:0) 0.96 ± 0.04 0.96 ± 0.15 4.73 ± 0.21 4.55 ± 0.36
  1. 3T3-L1 adipocytes were incubated with FA-DM (10 μmol/L C16-palmic acid, C16-palmitoleic acid, C18-stearic acid, C18-oleic acid, C18-linoleic acid, and C20-arachidic acid bound to 60 μmol/L FA-free BSA) for 30 min or 12 d. Cells were then subjected to transesterification and injected into a GC for FA analysis. The results are expressed as the percent of the total FA content (100%). The data are presented as the mean ± SEM of six independent wells.
  2. *P < 0.05; **P < 0.01.