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Table 3 FA composition of 3T3-L1 adipocytes overexpressing DGAT2 cultured with FA-DM

From: Effect of diacylglycerol acyltransferase 2 overexpression in 3T3-L1 is associated to an increase in mono-unsaturated fatty acid accumulation

Fatty acids

30 min

12 d

 

Control

DGAT2

Control

DGAT2

Myristic acid (C14:0)

2.70 ± 0.15

2.62 ± 0.49

2.84 ± 0.42

2.42 ± 0.29

Palmitoleic acid (C16:1)

2.67 ± 0.15

3.86 ± 0.33*

2.61 ± 0.17

3.54 ± 0.33*

Palmitic acid (C16:0)

39.11 ± 4.55

33.61 ± 2.104

31.17 ± 1.1003

29.23 ± 1.34

Linoleic acid (C18:2)

5.14 ± 0.38

6.63 ± 1.74

8.44 ± 0.29

11.38 ± 0.41**

Oleic acid (C18:1)

14.18 ± 1.19

12.31 ± 1.07

8.66 ± 0.32

16.47 ± 0.52**

Stearic acid (C18:0)

22.95 ± 2.33

26.45 ± 0.99

26.76 ± 0.42

22.54 ± 0.90**

Arachidonic acid (C20:4)

12.29 ± 1.88

13.56 ± 1.99

15.65 ± 0.46

9.86 ± 1.10**

Arachidic acid (C20:0)

0.96 ± 0.04

0.96 ± 0.15

4.73 ± 0.21

4.55 ± 0.36

  1. 3T3-L1 adipocytes were incubated with FA-DM (10 μmol/L C16-palmic acid, C16-palmitoleic acid, C18-stearic acid, C18-oleic acid, C18-linoleic acid, and C20-arachidic acid bound to 60 μmol/L FA-free BSA) for 30 min or 12 d. Cells were then subjected to transesterification and injected into a GC for FA analysis. The results are expressed as the percent of the total FA content (100%). The data are presented as the mean ± SEM of six independent wells.
  2. *P < 0.05; **P < 0.01.
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891