Figure 5

Effect of DGAT2 overexpression on adipogenic gene protein expression. The cells were incubated with FA-DM (DM containing 10 μmol/L. C₁₆-palmic acid, C₁₆-palmitoleic acid, C₁₈-stearic acid, C₁₈-oleic acid, C₁₈-linoleic acid, and C₂₀-arachidic acid bound to 60 μmol/L FA-free BSA). After 12 d of differentiation, total protein in mature adipocytes was extracted for western blotting. (A) Western blotting images; (B) Statistical analysis of protein expression using Image J software. Cells transfected with the empty pcDNA3.1(+) vector were control cells. Results are presented as the mean ± SEM of three independent cell preparations. *P < 0.05, **P < 0.01.