Figure 4

Effect of DGAT2 overexpression on adipogenic gene mRNA expression. The cells were incubated with FA-DM (DM containing 10 μmol/L C₁₆-palmic acid, C₁₆-palmitoleic acid, C₁₈-stearic acid, C₁₈-oleic acid, C₁₈-linoleic acid, and C₂₀-arachidic acid bound to 60 μmol/L FA-free BSA). After 12 d of differentiation, total RNA in mature adipocytes were isolated for quantitative PCR. Adipocytes transfected with the empty pcDNA3.1(+) vectors were control cells. Results are presented as the mean ± SEM of six independent cell preparations. *P < 0.05, **P < 0.01.