Table 2 Primer sequences for real-time reverse transcription-PCR 1
Gene | (5’–3’) | Accession number2 |
|---|---|---|
ALP (Forward) | GACATGACCTCCCAGGAAGA | XM_001504312.1 |
ALP (Reverse) | GCAGTGAAGGGCTTCTTGTC | |
GAPDH (Forward) | ATCACTGCCACCCAGAAGAC | NM_001163856 |
GAPDH (Reverse) | GTGAGCTTCCCATTCAGCTC | |
Osteocalcin (Forward) | GTGCAGAGTCTGGCAGAGGT | XM_001915727.1 |
Osteocalcin (Reverse) | CCAGCCAATGATCCAGGTAG | |
Osterix (Forward) | GCTCACTATGGCTCCAGTCC | XM_001494930.3 |
Osterix (Reverse) | AAGGTCACTGCCCACAGAGT | |
Runx2 (Forward) | CAGACCAGCAGCACTCCATA | XM_001502519.3 |
Runx2 (Reverse) | GCAGCATTCTGGAAGGAGAC | |
Type I Collagen (Forward) | TTGACCCTAACCAAGGATGC | AB070840.1 |
Type I Collagen (Reverse) | TTCTTGGCTGGGATGTTTTC | |
T-box3 (Forward) | GCATCCCTTTCTCGTCTCTG | XM_001914978 |
T-box3 (Reverse) | GACCATCTCGGTACCCCTCT |