Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites

Table 1 Composition of experimental diets

Items	Day 1-21	Day 22-42
Ingredient (%)
Corn	50.44	59.40
Soybean meal, 45%	27.88	22.00
Corn gluten meal, 58%	12.00	11.03
Soybean oil	5.03	3.57
Dicalcium phosphate phosphate	1.89	1.42
Limestone	1.25	1.31
Salt	0.50	0.50
Mineral premix^a	0.20	0.20
Vitamin premix^b	0.03	0.02
Choline chloride (50%)	0.10	0.16
Aureomycin (15%)	0.10	0.10
Ethoxyquin (33%)	0.03	0.03
L-lysine	0.45	0.24
DL-Methionine	0.10	0.02
Total	100.00	100.00
Calculated analysis
ME, Mcal/kg	3.20	3.20
CP (%)	23.00	20.00
Ca (%)	1.00	0.90
Available P (%)	0.45	0.35
Lysine (%)	1.25	1.00
Methionine (%)	0.50	0.38

^a supplied the following per kg complete diet: Cu, 8 mg; Zn, 75 mg; Fe, 80 mg; Mn, 100 mg; Se, 0.15 mg; I, 0.35 mg.
^b supplied the following (per kg complete diet): vitamin A, 12500 IU; vitamin D₃, 2500 IU; vitamin E, 30 IU; vitamin K₃, 2.65 mg; thiamine, 2 mg; riboflavin, 6 mg; vitamin B₁₂, 0.025 mg; biotin, 0.0325 mg; folic acid, 1.25 mg; pantothenic acid, 12 mg; niacin, 50 mg.

ISSN: 2049-1891