Figure 1
From: Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
Schematic representation of the stem-loop method for the study of miRNA expression. The reverse transcription of miRNA can be performed using a stem-loop RT primer that contains a universal reverse primer sequence on the loop and several specific bases at 3' end that are complementary to the 3'end of mature miRNA. The cDNA is amplified with specific forward and universal reverse primers. The amplification products can be detected by either (A) specific Taqman probe or (B) universal UPL (Universal ProbeLibrary) probe.