Table 1 Primer sequences for the target genes
Target genes | GenBank accession numbers | PCR products (bp) | Primer sequences |
|---|---|---|---|
β-actin | NM 205518 | 300 | F: 5′- tgcgtgacatcaaggagaag -3′ |
|  |  |  | R: 5′- tgccagggtacattgtggta -3′ |
LEPR | NM 204323 | 87 | F: 5′- gcatctctgcatctcaggaaaga -3′ |
|  |  |  | R: 5′- gcaggctacaaactaacagatcca -3′ |
SREBP-1c | NM 204126 | 104 | F: 5′- gcagaagagcaagtccctcaa -3′ |
|  |  |  | R: 5′- tcggcatctccatcacctc -3′ |
SREBP-2 | XM 416222 | 108 | F: 5′- cccagaacagcaagcaagg -3′ |
|  |  |  | R: 5′- gcgaggacaggaaagagagtg -3′ |
HMGCR | AB 109635 | 137 | F: 5’-ttggatagagggaagagggaag -3’ |
|  |  |  | R: 5’- ccatagcagaacccaccaga-3’ |
CYP7A1 | AB 109636 | 106 | F:5’- cattctgttgccaggtgatgtt -3’ |
|  |  |  | R:5’- gctctctctgtttcccgcttt -3’ |