Fig. 6

Role of the Nrf2/Keap1 pathway in the effects of Lut on ROS, GSH, and mitochondrial function in porcine PA embryos. A Fluorescent images of embryos stained with CM-H2DCFDA in the indicated groups. Scale bar = 100 µm. B Quantification of ROS fluorescence intensity in the indicated groups (n = 36 per group). C Fluorescent images of embryos stained with CMF2HC in the indicated groups. Scale bar = 100 µm. D Quantification of GSH fluorescence intensity in the indicated groups (n = 38 per group). E Fluorescent images of embryos stained with MitoTracker in the indicated groups. Scale bar = 100 µm. F Quantification of MitoTracker fluorescence intensity in the indicated groups (n = 42 per group). G Fluorescent images of embryos stained with TMRM in the indicated groups. Scale bar = 50 µm. H Quantification of TMRM fluorescence intensity in the indicated groups (n = 24 per group). The data are derived from three independent experiments, and means with similar superscripts do not differ (P > 0.05)