Fig. 5

Role of the Nrf2/Keap1 pathway in the effects of Lut on the early development of porcine PA embryos. A Representative bright-field images of blastocysts cultured in the indicated groups. Scale bar = 200 µm. B, C Quantification of the cleavage rate and blastocyst formation rate in the indicated groups (Con; n = 180, Bru; n = 180, Bru + Lut; n = 181). D TUNEL assay of blastocysts in the indicated groups. Embryos were stained for TUNEL (green, white arrow) and nuclei (blue). Scale bar = 50 µm. E-G Quantification of the total cell number, number of apoptotic cells, and proportion of apoptotic cells in the indicated groups (n = 21 per group). The data are derived from five independent experiments, and means with similar superscripts do not differ (P > 0.05)