Fig. 3

Effects of Lut on mitochondrial functions in porcine PA embryos. A Fluorescent images of D2 embryos and D6 blastocysts stained with MitoTracker in the indicated groups. Scale bar = 100 µm. B Quantification of MitoTracker fluorescence intensity in the indicated groups (D2; n = 51 per group, D6; n = 26 per group). C Fluorescent images of D2 embryos and D6 blastocysts stained with TMRM in the indicated groups. Scale bar = 50 µm. D Quantification of TMRM fluorescence intensity in the indicated groups (D2; n = 23 per group, D6; n = 24 per group). The data are derived from three independent experiments, and means with similar superscripts do not differ (P > 0.05)