Fig. 2

Effects of Lut on intracellular reactive oxygen species (ROS) and glutathione (GSH) levels in porcine PA embryos. A Fluorescent images of D2 embryos and D6 blastocysts stained with CM-H2DCFDA in the indicated groups. Scale bar = 100 µm. B Quantification of ROS fluorescence intensity in the indicated groups (D2; n = 48 per group, D6; n = 26 per group). C Fluorescent images of D2 embryos and D6 blastocysts stained with CMF2HC in the indicated groups. Scale bar = 100 µm. D Quantification of GSH fluorescence intensity in the indicated groups (D2; n = 36 per group, D6; n = 24 per group). The data are derived from three independent experiments, and means with similar superscripts do not differ (P > 0.05)