Palm oil protects α-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids

In ruminants, dietary C18:3n-3 can be lost through biohydrogenation in the rumen; and C18:3n-3 that by-passes the rumen still can be lost through oxidation in muscle, theoretically reducing the deposition of C18:3n-3, the substrate for synthesis of poly-unsaturated fatty acids (n-3 LCPUFA) in muscle. In vitro studies have shown that rumen hydrogenation of C18:3n-3 is reduced by supplementation with palm oil (rich in cis-9 C18:1). In addition, in hepatocytes, studies with neonatal rats have shown that cis-9 C18:1 inhibits the oxidation of C18:3n-3. It therefore seems likely that palm oil could reduce both rumen biohydrogenation of C18:3n-3 and muscle oxidation of C18:3n-3. The present experiment tested whether the addition of palm oil to a linseed oil supplement for goat kids would prevent the losses of C18:3n-3 and thus improve the FA composition in two muscles, Longissimus dorsi and Biceps femoris. To investigate the processes involved, we studied the rumen bacterial communities and measured the mRNA expression of genes related to lipid metabolism in Longissimus dorsi. Sixty 4-month-old castrated male Albas white cashmere kids were randomly allocated among three dietary treatments. All three diets contained the same ingredients in the same proportions, but differed in their fat additives: palm oil (PMO), linseed oil (LSO) or mixed oil (MIX; 2 parts linseed oil plus 1 part palm oil on a weight basis). Compared with the LSO diet, the MIX diet decreased the relative abuandance of Pseudobutyrivibrio, a bacterial species that is positively related to the proportional loss rate of dietary C18:3n-3 and that has been reported to generate the ATP required for biohydrogenation (reflecting a decrease in the abundance of rumen bacteria that hydrogenate C18:3n-3 in MIX kids). In muscle, the MIX diet increased concentrations of C18:3n-3, C20:5n-3, C22:6n-3, and n-3 LCPUFA, and thus decreased the n-6/n-3 ratio; decreased the mRNA expression of CPT1β (a gene associated with fatty acid oxidation) and increased the mRNA expression of FADS1 and FADS2 (genes associated with n-3 LCPUFA synthesis), compared with the LSO diet. Interestingly, compared to Longissimus dorsi, Biceps femoris had greater concentrations of PUFA, greater ratios of unsaturated fatty acids/saturated fatty acids (U/S), and poly-unsaturated fatty acids/saturated fatty acids (P/S), but a lesser concentration of saturated fatty acids (SFA). In cashmere goat kids, a combination of linseed and palm oils in the diet increases the muscle concentration of n-3 LCPUFA, apparently by decreasing the relative abundance of rumen bacteria that are positively related to the proportional loss rate of dietary C18:3n-3, by inhibiting mRNA expression of genes related to C18:3n-3 oxidation in muscle, and by up-regulating mRNA expression of genes related to n-3 LCPUFA synthesis in muscle, especially in Longissimus dorsi.


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Conclusions: In cashmere goat kids, a combination of linseed and palm oils in the diet increases the muscle concentration of n-3 LCPUFA, apparently by decreasing the relative abundance of rumen bacteria that are positively related to the proportional loss rate of dietary C18:3n-3, by inhibiting mRNA expression of genes related to C18:3n-3 oxidation in muscle, and by up-regulating mRNA expression of genes related to n-3 LCPUFA synthesis in muscle, especially in Longissimus dorsi.

Background
The long chain n-3 poly-unsaturated fatty acids (n-3 LCPUFA), such as C20:5n-3 and C22:6n-3, have a wide range of biological effects that have long been believed to be beneficial for human health [1,2]. An important dietary source of n-3 LCPUFA is meat from ruminants, so there have been many attempts to increase the muscle concentration of n-3 LCPUFA in livestock by feeding dietary supplements [3]. In theory, this outcome could be accomplished by feeding the animals with linseed oil because it is rich in C18:3n-3, the substrate for n-3 LCPUFA synthesis [4]. However, dietary C18:3n-3 can be hydrogenated extensively by rumen bacteria [5], potentially leading to a reduction in post-ruminal C18: 3n-3 flow. This problem might be overcome by feeding oils rich in cis-9 C18:1, as revealed by an in vitro rumen study in which palm oil was added to a basal dietafter 24 h incubation, it decreased C18:3n-3 biohydrogenation but enhanced cis-9 C18:1 biohydrogenation, in a doseresponsive manner [6]. However, the ruminal microbes involved in this process have not been identified.
The next challenge is that the ruminal "by-pass" C18: 3n-3 can be oxidized in the various tissues and organs that are involved in fatty acid (FA) metabolism [7], leading to a reduction in the tissue deposition of C18:3n-3, and therefore the local synthesis of n-3 LCPUFA in muscle.
On the other hand, susceptibility to oxidative stress in mammals is increased by consumption of dietary n-3 PUFA [8,9]. In the liver, oxidative stress can reduce the gene expression and catalytic activity of Δ-5 and Δ-6 desaturases (FADS1 and FADS2), the most relevant enzymes in n-3 LCPUFA biosynthesis, thereby reducing the tissue levels of n-3 LCPUFA [10]. Again, in vitro studies have presented a possible solutionin a study with rat hepatocytes, the oxidative stability of C18:3n-3 was improved by blending C18:3n-3 with cis-9 C18:1 [11].
Together, these observations suggest that more substrate and desaturases would become available for the synthesis of n-3 LCPUFA in vivo if the biohydrogenation and oxidation of C18:3n-3 could be reduced by blending linseed oil with palm oil in ruminant diets. Moreover, any changes in biohydrogenation pathways in the rumen would probably be explained by changes in the bacterial communities [5]. Therefore, using cashmere kids, we tested whether diets supplemented with a blend of linseed and palm oils increases the muscle concentration of n-3 LCPUFA more than linseed oil alone, and whether this outcome is mediated by i) a reduction in the abundance of bacteria that hydrogenate C18:3n-3 in the rumen; ii) a decrease in mRNA expression of CPT1β (a gene related to FA oxidation), and iii) an up-regulation of the mRNA expression of FADS1 and FADS2 in muscle.

Animals, diets and feeding management
This study was conducted on the Inner Mongolia White Cashmere Goat Breeding Farm, Wulan Town, Etuoke Banner, Ordos City, Inner Mongolia Autonomous Region, China (39°12′N; 107°97′E). Sixty 4-month-old castrated male kids (average body weight 18.6 ± 0.1 kg) were selected and randomly allocated among three groups, each of which comprised four units of five kids. All three diets contained the same ingredients in the same proportions, but their fat additives differed (Table 1): palm oil (PMO), linseed oil (LSO) or mixed oil (MIX; linseed oil blended with palm oil in a 2:1 ratio based on weight, providing 4.7% fat, 35% C18:3n-3 and 20% cis-9 C18:1). For blending, palm oil (Jiali, Shanghai, China) and linseed oil (Mengyue Xiang Biotechnology Co., Ltd., Inner Mongolia, China) were removed from frozen storage and placed in direct sunlight (about 25°C) to defrost, then mixed in a stainless steel vessel.
The diets were prepared by manually blending the oil thoroughly into the ground concentrate to ensure homogenous distribution throughout the ration. The diets were prepared fresh twice each day and were offered as a total mixed ration (TMR) in two equal meals at 08:30 and 16:30 h. The kids were given free access to drinking water. The diets were fed for 104 d, consisting of 14 d for adaptation followed by 90 d of treatment. The treatment period was divided into early (1-30 d), middle (31-60 d) and late periods (61-90 d) so the amount and composition of diet offered could be increased to meet the needs of cashmere kids as they grow, according to the feeding  Table 1).

Sampling and slaughtering procedures
To estimate dry matter intake for five kids in each pen, refusals were collected and weighed 30 min before each feeding, at 08:00 h daily. After weighing, the refusals were evenly sprinkled on the surface of the fresh TMR and were re-fed to the kids. The amount of feed offered was adjusted daily in the morning to ensure a 10% refusal (as fed basis). Samples of TMR were collected at the beginning of each period and stored at − 20°C for chemical analysis. At the end of the experiment, two kids from each experimental unit (total 8 per treatment) were randomly selected and slaughtered by exsanguination. Before slaughter, the kids were prevented from consuming feed for 24 h and from drinking for 2 h. Immediately after death, the rumen was dissected and digesta was squeezed through two layers of cheesecloth. Left Longissimus dorsi and left Biceps femoris were collected. Subsamples of rumen liquid (500 mL) and muscle (100 g) were snap frozen in liquid N 2 and stored at − 80°C until analysis.

Chemical analyses Analysis of feed
Samples of dietary ingredients were analyzed for DM (method 930.15), ether extract (method 920.39), CP (N × 6.25; method 984.13), calcium and phosphorous (method 935.13) according to AOAC [13]. Neutral detergent fibre and ADF were determined according to the methods described by Van Soest et al. [14] with an Ankom 220 Fiber Analyser (Ankom Co., USA) and were expressed inclusive of residual ash. Heat stable amylase was not used in the NDF determination.

Measurement of FA
Fatty acid methyl esters were produced from samples of feed, plasma, muscle, and rumen liquid, according to the method of O'Fallon et al. [15], and were analyzed as described previously [16].

RNA extraction and real-time PCR
For Longissimus dorsi only, total RNA was extracted from 0.5 g samples of frozen tissue using the RNAiso Reagent (TaKaRa, Dalian, China) according to the manufacturer's recommendations. The concentration, purity and integrity of the RNA were assessed by 2% agarose gel electrophoresis and a microplate reader (Synergy H4, BioTek, USA) at 260/280 nm (OD260/OD280 = 1.8-2.0). Synthesis of first-strand cDNA and quantitative realtime PCR were performed as described previously [16,17]), with the same primer pair sequences, and the same three gene references (β-2-microglobulin, tyrosine 3-monooxygenase, β-actin). The primers used are presented in Supplementary Table S1. The efficiency of PCR amplification for each gene was calculated with the standard curve method (E = 10 − 1 /slope). The efficiency of PCR amplification for all genes was between 0.98-0.99 (Supplementary Table S1). The 2 −ΔΔCt method was used to analyze the qPCR data [18]. The qPCR data were normalized using the geometric mean Ct of the three reference genes [19].

Metagenomic analyses DNA extraction
Microbial DNA was extracted from rumen samples from six of the eight slaughtered kids in each group, using the E.Z.N.A.® soil DNA Kit (Omega Biotek, Norcross, GA, U.S.) according to the manufacturer's protocols. DNA integrity was evaluated using 1% agarose gel electrophoresis. The DNA was diluted to 1 ng/μL using sterile water. The extraction and the metagenomic analyses were conducted in Inner Mongolia Agriculture University.

Hiseq sequencing and data analysis
Polymerase chain reaction was used to amplify the V4 region of the bacterial 16S rRNA gene using the universal primers 515F (5´-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5´-GGACTACHVGGTWTCTAAT-3′; with the barcode [20]. The forward primer contained 6base barcode sequences. The reaction was carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). The PCR reaction mixture (30 μL) contained 10 μL DNA template, 15 μL of Phusion Master Mix (2×), 1.5 μL of each primer (total 6 μmol/L and 2 μL of double-distilled H 2 O. The PCR was performed under the following conditions: 98°C for 1 min, followed by 30 cycles of 98°C for 10 s, 50°C for 30 s, and 72°C for 30 s, and a final elongation step of 72°C for 5 min. The same volume of 1× loading buffer (contained SYB green) was mixed with the PCR products and the mixture was subjected to electrophoresis on 2% agarose gel. Samples with a bright main strip between 400 and 450 bp were chosen for further analysis. The PCR products were mixed in equimolar amounts and then purified with a Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA), following the manufacturer's recommendations, and index codes were added. Library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina HiSeq 2500 platform and 250 bp paired-end reads were generated. The generated raw sequences were processed using FLASH and Trimmomatic to merge the paired-end sequences and remove low quality reads with the following criteria: i) The reads were truncated at any site receiving an average quality score < 20 over a 50-bp sliding window; ii) Primer matching allowed 2-nucleotide mismatching, and reads containing ambiguous bases were removed; iii) Sequences with an overlap longer than 10 bp were merged according to their overlap sequence. Operational taxonomic units (OTUs) were clustered with a 97% similarity cut-off using UPARSE (version 7.1 http://drive5.com/uparse/) and chimeric sequences were identified and removed using UCHIME. The taxonomy of each 16S rRNA gene sequence was analyzed with the RDP Classifier algorithm (http://rdp.cme.msu.edu/) against the Silva 128 16S rRNA database (Release128 http://www.arb-silva.de) using a confidence threshold of 70%. Bacterial diversity was measured using the QIIME pipeline based on the OTUs [21]. To eliminate variation among individual kids and thus allow all samples to be compared at the same OTU sequence number, OTU abundance information was normalized using a standard sequence number corresponding to the sample with the least sequences. Subsequent analyses were performed on this output-normalized data.

Statistical analysis
The data for muscle FA composition were analyzed using PROC MIXED of SAS (version 9.2, SAS Institute Inc., Cary, NC). The MIXED statistical model used for analysis was y ijkl = μ + L i + E j + LE ij + A ijk + T l + TL il + TE jl + TLE ijl + ε ijkl where y ijkl was the dependent, continuous variable, μ is the overall mean, L i was the fixed effect of diet (i = palm oil, linseed oil or mixed oil), E j was the fixed effect of tissue (j = longissimus dorsi or biceps femoris), A ijk was the random effect of the k th pen in the ij th combination of diet and tissue, T l was the random effect of pen, the two-and three-way interactions of diet, tissue and pen were all considered fixed effects, and ε ijkl was the residual error. Pen was considered as the experimental unit. Tissue was a repeated measurement. Least square means were compared using LSD and statistical differences were declared significant at P < 0.05, and tendencies are discussed at 0.05 ≤ P < 0.10.
Dry matter intake, rumen FA, plasma FA, and mRNA expression were analyzed using the MIXED procedure. The statistical model included treatment as fixed effects, and pens were added to the model as random effects. Specifically, the model used to study DMI consider 4 replicates (number of pens), each with 1 observation; rumen FA, plasma FA, and mRNA expression consider 4 replicates (number of pens), each with 2 observations (number of goats), for each treatment. The effects of fixed factors were declared significant at P < 0.05, and tendencies are discussed at 0.05 ≤ P < 0.10.
Multivariate analysis was carried out using R software with the nonparametric MANOVA (Adonis) add-on. Adonis was performed on the Weighted Unifrac distances to assess the significance of differences in bacterial community structure across treatments at a significance level of α = 0.05 based on 9999 possible permutations. Non-metric multidimensional scaling (nMDS) plots were constructed to visualize the data. The ternary plot was created with GGTERN. For indices of bacterial diversity, ANOVA and post hoc, Tukey HSD tests were carried out. The results are presented as the mean and standard error of the mean (SEM). Data means were considered significantly different at P < 0.05.
The rates of reduction in C18:3n-3 and cis-9 C18:1 from dietary values (d 61-90) to rumen values, were considered to reflect hydrogenation [6,[22][23][24]. The rate of increment in the proportion of C18:0 from diet (d 61-90) to rumen was considered to reflect synthesis. The six rumen samples used for analysis of bacterial community structure in each group were also used to calculate the rates of reduction and increment in FAs. ANOVA and post hoc Tukey HSD tests were carried out. Spearman correlation analysis was used to relate the abundance of the top 45 bacterial genera and the rate of hydrogenation of dietary C18:3n-3 and cis-9 C18:1, and the rate of synthesis of C18:0, using R (pheatmap package). Only correlations with P < 0.05 for the linear model were considered as significant.

Muscle FA composition
There were significant interactions (P < 0.05) between oil type and muscle for concentrations of C21:0, C17:1, C18:2n-6t, C18:3n-6, C20:2n-6, C18:3n-3, C22:6n-3, and the n-6/n-3 ratio (Table 3). For all dietary treatments, the values for C18:2n-6t concentration and n-6/n-3 ratio were significantly greater (P < 0.05) in Biceps femoris than in Longissimus dorsi. Values for C18:3n-6 concentration and C20:2n-6 in Biceps femoris were significantly lower (P < 0.05) with MIX than with the other treatments. The highest C21:0 concentration was observed in Biceps femoris with LSO treatment. The lowest C17:1 concentration was observed in Longissimus dorsi with PMO treatment. The lowest C18:3n-3 concentrations were observed in both muscles with PMO treatment, while the highest C18:3n-3 concentrations were observed in Biceps femoris with MIX treatment, and in Biceps femoris with LSO treatment. The concentration of C22:6n-3 was significantly higher in Longissimus dorsi with LSO treatment, and in Longissimus dorsi with MIX treatment (P < 0.05). Significant main effects are also shown in Table 3. Compared with Biceps femoris, total fat content tended to be greater in Longissimus dorsi (P = 0.066). Dietary supplementation with LSO or MIX increased the muscle concentrations of C18:3n-3 and n-3 PUFA (P < 0.05), but decreased the muscle concentration of C16:1 (P < 0.05) and the n-6/n-3 ratio (P < 0.05), and tended to decrease the muscle concentration of MUFA (P = 0.066), compared with PMO. The muscle concentrations of those FAs did not differ (P ≥ 0.10) between kids fed the LSO and MIX diets.
Muscle concentrations of C20:5n-3 were greater (P < 0.05) and concentrations of n-3 LCPUFA tended to be greater (P = 0.098), in MIX kids compared with PMO kids, but the value for LSO kids did not differ from those for either PMO or MIX kids (P ≥ 0.10). Diets containing PMO or LSO alone reduced the concentration of C22: 6n-3 compared with the MIX diet (P < 0.05). Muscle concentration of C22:6n-3 did not differ between kids fed LSO or PMO alone (P ≥ 0.10). Muscle concentrations of C15:0, C18:2n-6t, C21:0, and C20:1 were greater in LSO-fed kids compared with PMO and MIX-fed kids (P < 0.05), but did not differ (P ≥ 0.10) between PMO and MIX kids. Muscle concentrations of C18:3n-6 and C20:2n-6 were lower in MIX-fed kids than in LSO-fed kids (P < 0.05). The C18:3n-6 and C20:2n-6 values for PMO kids did not differ (P ≥ 0.10) from those for either LSO or MIX kids. Compared with PMO-fed kids, muscle concentration of C22:2n-6 were lower in LSO-fed kids (P < 0.05), but the value for MIX kids did not differ from those for either PMO kids or LSO kids (P ≥ 0.10).

mRNA expression
The relative mRNA expression of genes in Longissimus dorsi is presented in Fig. 1. In comparison to PMO, mRNA expression of fatty acid synthetase (FAS) was greater (P < 0.05) with both the LSO and MIX treatments, with no difference (P ≥ 0.10) between MIX and LSO kids. In comparison to the LSO treatment, mRNA expression of carnitine palmitoyltransferase I (CPT1β) was lower with the PMO and MIX treatments (P < 0.05), and mRNA expression of CPT1β was greater with the MIX treatment than with the PMO treatment (P < 0.05). Kids fed the mixed oil diet showed greater mRNA expression of FADS1 and FADS2 than PMO-fed kids, but  LSO-fed kids did not differ (P ≥ 0.10) from either PMOfed or MIX-fed kids.

Bacterial diversity Sequencing coverage and bacterial diversity
A total of 1,336,287 reads were generated after quality control and chimera removal, resulting in an average of 74,238 reads per sample, with sequence numbers per sample ranging from 51,620 to 85,348 (median 74,632). A total of 418 unique OTUs that could be taxonomically classified to genus level were identified across all samples. The OTU rarefaction curves of the bacterial communities in the ruminal digesta show that the sampling effort was sufficient to estimate bacterial diversity (Supplementary Fig. S1). Alpha diversity indices (Table 5) indicated that supplementation did not significantly affect OTU number, ACE, Chao, Simpson, Shannon, and coverage indices (P ≥ 0.10).

Bacterial community
As reflected by nMDS using the weighted Unifrac similarity metric, the samples clustered according to the dietary treatments (Fig. 2). There is a clear separation between PMO and LSO (Adonis analysis, P < 0.001), whereas the points for the MIX animals were spread.

Spearman correlation analysis
The 45 most abundant genera represent 91% (PMO), 89% (LSO), and 92% (MIX) of the total microbiome. Spearman correlation analysis was conducted between the abundance of the top 45 genera and the proportional loss rate of fatty acids (C18:3n-3 and cis-9 C18:1), or the proportional increment rate of fatty acid (C18:0) from diet to rumen (Fig. 3). The threshold |R| > 0.4 is considered as a significant Spearman correlation. Among the top 45 genera, an unclassified genus was clustered among the genera belonging to the Bacteroidetes phylum, and was labelled 'unclassified_k_norank' by the phylogenetic analysis software (Supplementary Fig. S2).
The results indicated that relative abundance of the unclassified genera in Bacteroidetes, Succinivibrio, and Succinivibrionaceae UCG-002 were positively correlated with the rate of reduction in the proportion of cis-9 C18:1 from diet to rumen, but negatively correlated with the rate of reduction in the proportion of C18:3n-3 from diet to rumen. The relative abundance of Pseudobutyrivibrio was positively correlated with the rate of reduction in the proportion of C18:3n-3, but negatively correlated with the rate of reduction in the proportion of cis-9 C18:1. For the relative abundance of Ruminococcus_2, there was a positive correlation only with the rate of increase in the proportion of C18:0 from diet to rumen.

Microbial composition analysis
Collectively, 26 bacterial phyla, 183 families, 418 genera, and 789 species were identified in the rumen samples. Independent of diets, Bacteroidetes, Firmicutes, Proteobacteria, and Verrucomicrobia were the dominant phyla ( Supplementary Fig. S3), comprising about 89% of average relative abundance. The most abundant 20 families are shown in Table 6, representing 92% (PMO), 88% (LSO), and 94% (MIX) of the total microbiome. The relative abundance of Bacteroidales_BS11_gut_group was greater in PMO-fed kids than in LSO-fed and MIXfed kids (P < 0.05), but did not differ (P ≥ 0.10) between LSO-fed and MIX-fed kids. The relative abundance of Veillonellaceae and Acidaminococcaceae was greater in LSO-fed kids than in PMO-fed and MIX-fed kids (P < 0.05), but did not differ between PMO-fed and MIX-fed kids (P ≥ 0.10). Compared with PMO-fed kids, the relative abundance of Bacteroidales_S24-7_group was greater in MIX-fed kids (P < 0.05), but values for LSOfed kids did not differ (P ≥ 0.10) from those in either the PMO-fed or MIX-fed kids. The centre of the ternary plot (Fig. 4) shows the core microbiome (high density of circles) across the PMO, LSO, and MIX treatments. Ternary plot analysis focuses

Discussion
The LSO diet contained more C18:3n-3 than the MIX diet, but only the MIX diet increased muscle n-3 LCPUFA concentration to levels greater than those observed with PMO diet. These results are consistent with the literature for goats [3,25] showing that the C22:6n-3 content of muscle and fat increased as dietary C18:3n-3 content increased with inclusion of palm kernel cake in the diet. For cattle, two observations are relevant: i) the muscle concentration of C22:6n-3 was increased in Longissimus dorsi by feeding a diet containing both palm oil and linseed oil, but not diets containing only palm oil diet or linseed oil [26]; ii) a supplement of extruded linseed oil increased the muscle contents of C18:3n-3, but not C22:6n-3 [4]. Our observations support the hypothesis that these outcomes are the result of two processes, one in the rumen and the other in muscle tissue.

Mixed oil decreases the hydrogenation of dietary C18:3n-3 in rumen
In agreement with a previous study [6], the MIX diet decreased the proportional loss rate of C18:3n-3 but increased the proportional loss rate of cis-9 C18:1 compared with LSO diet, leading to an increased postruminal C18:3n-3 flow, an expectation that is consistent with the similar plasma concentrations of C18:3n-3 in LSO and MIX kids.The proportional loss rate of FA reflects FA hydrogenation in the rumen [6,[22][23][24], evidenced by the decreased C18:3n-3 hydrogenation but increased cis-9 C18:1 hydrogenation in MIX diet compared with LSO diet. The relative abundance of Pseudobutyrivibrio was positively correlated with the rate of Table 4 Fatty acid profiles (percentage of total identified fatty acid methyl esters) in plasma of cashmere goat kids fed diets with oil supplements (Continued) Except for Pseudobutyrivibrio [27], there is no direct evidence that unclassified genus in Bacteroidetes, Succinivibrionaceae UCG-002, and Succinivibrio are involved in FA hydrogenation, but these bacteria are thought to provide the energy or hydrogen needed for FA hydrogenation [28][29][30][31][32][33][34][35]. Further studies are needed to confirm the function of FA hydrogentaion of these bacteria. At family level, Acidaminococcaceae is also involved in the hydrogenation of C18:3n-3 [36]. Veillonellaceae become the most important ruminal bacteria in goats fed a diet supplemented with linseed oil [37], suggesting that they also play an important role in the hydrogenation of dietary C18: 3n-3, perhaps because they can provide hydrogen and energy [38].
Ternary plot analysis showed that MIX-fed kids had a lower abundance of Pseudobutyrivibrio, but greater abundance of Succinivibrionaceae UCG-002, unclassified genus in Bacteroidetes, and Succinivibrio, than LSO-fed kids. The relative abundances of the 20 most abundant bacteria (family level) showed that MIX-fed kids had a lower abundance of Acidaminococcaceae and Veillonellaceae, but greater abundance of Bacteroidales_S24-7_ group, than LSO-fed kids. All of these outcomes would probably lead to a decrease in the proportional loss rate of C18:3n-3 and an increase in the proportional loss rate of cis-9 C18:1 in MIX-fed kids compared with LSO-fed kids, as observed in vitro [6].
Increases in C18:0 are a consequence of the extensive biohydrogenation in the goat rumen [5]. The abundance of Ruminococcus_2 was positively correlated with the proportional increment rate in C18:0 in the present study, which indicated that Ruminococcus_2 is related to hydrogenation, consistent with the ability of H 2 production [39]. MIX-fed kids had the highest abundance of Ruminococcus_2, consistent with the high proportion of rumen C18:0 in MIXfed kids. However, PMO-fed kids had the lowest abundance of Ruminococcus_2, but the highest proportional increment rate in C18:0. The implication is that PMO-kids had a high abundance of other bacteria that are responsible for the proportional increment rate of C18:0. The inclusion of different oils in the diet did not influence bacterial richness or diversity, as reported by other laboratories [40], with Bacteroidetes, Firmicutes, Proteobacteria, and Verrucomicrobia remaining the dominant phyla for all diets, as seen in other studies with goats and sheep [5,40].
The current study used proportional loss rate of FA to reflect FA biohydrogentation, and proportional increment rate in FA to reflect FA synthesis. Future research should consider more accurate calculation of the hydrogenation of FA and synthesis of FA, the passage rate, rumen volume, intake of FA, and rumen FA concentration.  Mixed oil decreases the expression of genes related to FA oxidation and increases n-3 LCPUFA content in muscle The key enzyme involved in oxidation of FA is CPT1β [41] and the most relevant enzymes in n-3 LCPUFA biosynthesis are FADS1 and FADS2 [10]. In the present study, the MIX diet decreased mRNA expression for CPT1β in muscle, compared with the LSO diet, thereby probably decreasing the production of the enzyme and the oxidation of C18:3n-3, helping explain the increase in the muscle concentration of C18:3n-3. Moreover, if less C18:3n-3 is oxidized, more substrate would be made available for n-3 LCPUFA synthesis, and up-regulation of mRNA expression of FADS1 and FADS2 would be observed, leading to an increase in the conversion of C18: 3n-3 to C20:5n-3 and C22:6n-3. Our observations are also consistent with previous studies in the goat, where a linseed oil supplement increased mRNA expression of CPT1β in adipose tissue [16], and in the rabbit where mRNA expression of CPT1 in liver increased as dietary C18:3n-3 content increased [42], and palm oil decreased the TBA-reactive substances thereby improving the lipid stability of PUFA- Fig. 3 Heat maps showing correlations between the relative abundance of sequences assigned to each bacterial genus and hydrogenation rate of fatty acids (C18:3n-3 and cis-9 C18:1) in the rumen of kids fed palm oil, linseed oil and mixed oil. Spearman correlation coefficients (r) are shown in different colors: r < 0 (blue), r = 0 (white) and r > 0 (red). * correlation significant at 0.01 < P ≤ 0.05; ** correlation significant at 0.001 < P ≤ 0.01 modified animal products (meat, egg and liver) [43].Taking all these findings into account, we conclude that combining linseed oil with palm oil offers a new approach for increasing the content of C18:3n-3 in goat meat, and oils with a high content of PUFA can be stablised in vivo by blending them with oils of high cis-9 C18:1 content, as originally suggested by Emmison et al. [11].

The effects of different dietary oils on the predominant FAs in goat muscle
We found the predominant FAs in goat muscle to be C16:0 and C18:0 as SFAs, cis-9 C18:1 as MUFAs, and C18:2n-6c as PUFAs, as reported by Ebrahimi et al. [3]. Profiles of C16:0 in the rumen, plasma, and liver also reflect the muscle profiles, with the diets ranking PMO ≥ MIX ≥ LSO, an observation from another study (Wang et al., unpublished), although muscle values did not differ significantly among these three treatments in the present study. Annison and Bryden [44] suggested that tissue C16:0 is derived from a combination of de novo synthesis and extraction from circulating plasma. Fatty acid synthetase (FAS) plays a central role in de novo lipogenesis in animals by catalyzing all the reactions involved in the conversion of acetyl-coA and malonyl-CoA to palmitate [45]. Increased mRNA expression of FAS in the muscle of LSO-fed and MIX-fed kids is assumed to increase the C16:0 concentration in muscle, thus nullifying the effect of the diet and explaining the lack of difference among the three groups in the muscle concentration of C16:0. In addition, ELOVL6 activity elongates C16:0 and cis-9 C16:1 to C18:0 and cis-9 C18: 1 [46], whereas SCD desaturates C18:0 to cis-9 C18:1 [47]. The results of the present study suggest that the lack of difference in mRNA expression of ELOVL6 and SCD1 in muscle among the three groups would explain the similar concentrations of C16:1, C18:0, and cis-9 C18:1. Again, these observations agree with others, using various tissues, showing that linseed oil does not affect SCD1, ACC or ELOVL6 [48,49], but increases the mRNA expression of FAS [9,50].

Difference in FA composition between muscle types
In sheep, Biceps femoris typically has a lower lipid content than Longissimus dorsi whereas a muscle containing low concentrations of lipid would have a greater proportion of functional FA, such as PUFA [51,52]. These observations explain why the concentration of PUFA was greater in Biceps femoris than in Longissimus dorsi in the present study. Working with bovine muscle, Talmant PMO palm oil diet, LSO linseed oil diet, MIX mixed oil diet a,b Means within the same row followed by the same superscript letters are not significantly different at P < 0.05 (n = 6 for each mean) et al. [53] found that Longissimus dorsi has a greater glycolytic activity than Biceps femoris, implying greater production of ATP, an essential driver of de novo synthesis of SFA. In the present study, SFA concentration was greater in Longissimus dorsi than in Biceps femoris, and there was proportionately less n-6 PUFA in Longissimus dorsi than in Biceps femoris. These observations suggest that a greater glycolytic activity and a lesser mitochondrial oxidative activity lead to a lower n-6/n-3 ratio in Longissimus dorsi compared with Biceps femoris. This hypothesis is supported by the observation in cattle that Biceps femoris has a greater content of PUFA, P/S, and n-6/n-3 ratios, but a lesser content of SFA, compared with Longissimus dorsi [52].
The interaction between oil type and muscle on n-3 PUFAs and n-6 PUFAs We observed that C18:3n-3 concentration was affected by both oil type and muscle type, with a lower concentration in both muscles with PMO treatment and a higher concentration in Biceps femoris with all oil treatments (expect for PMO). These observations agree with other studies showing that C18:3n-3 concentration is greater in Biceps femoris than in Longissimus dorsi in goats [54], and that dietary palm oil decreases C18:3n-3 concentrations in liver, adipose, and muscle of piglets, compared with linseed oil [55]. In mammals, C18:3n-3 is the subtrate for the synthesis of C22:6n-3 [4]. In Biceps femoris with both linseed-based treatments (LSO and MIX), the C18:3n-3 concentration was greater than in Longissimus dorsi with any treatment but, for C22:6n-3 concentration, the opposite outcome was observed. These observations suggest that Biceps femoris has a poor ability to synthesise C22:6n-3 from C18:3n-3 compared with Longissimus dorsi, as previously reported for goat meat [54]. Studies also demonstrated a higher C18: 2n-6 content in Biceps femoris than in Longissimus dorsi [54,56]. In the present study, we observed a higher C18: 2n-6t concentratio in Biceps femoris than in Longissimus dorsi, independently of oil treatment, leading to a higher n-6/n-3 ratio in Biceps femoris than in Longissimus dorsi.