Fig. 5
Sirtuin 3 overexpression alleviates BHBA-induced mitochondrial dysfunction in fGCs. A Representative images of the mitochondrial morphology of fGCs after the overexpression of Sirt3 with BHBA treatment by transmission electron microscopy (scale bars = 0.5 μm). Normal form mitochondria are marked with blue arrows, abnormal form mitochondria are marked with red arrows, and autolysosomes are marked with stars. B The abnormal rate of mitochondria was statistically analyzed in five images for each treatment group (n = 5). C–D Analysis of MPTP detection and fluorescence intensity after the overexpression of Sirt3 with BHBA treatment (scale bars = 20 μm, n = 8). E–F Representative images and fluorescence intensity analysis showing intracellular Ca2+ levels in different groups (scale bars = 100 μm, n = 5). G–H Representative images and fluorescence intensity analysis of mitochondrial staining in different groups (scale bars = 25 μm, n = 8). For Fig. 5B, D, F and H, we used one-way ANOVA for significant difference analysis (the different lowercases represent P < 0.05)