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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Sirtuin 3 regulation: a target to alleviate β-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells

Fig. 1

β-Hydroxybutyric acid-induced apoptosis in fGCs. A Representative images of FSHR staining in fGCs (scale bars = 25 μm). B Schematic protocol for BHBA treatment. C Cell viability of fGCs under different concentrations of BHBA stimulation (n = 6). DE Western blot analysis of BAX, BCL2, cleaved-caspase3, and cleaved-caspase9 in the control, BHBA-1.2 mmol/L and BHBA-2.4 mmol/L groups (n = 3). FG Apoptosis of fGCs in the control and BHBA-2.4 mmol/L treated groups were analyzed by flow cytometry after stimulation of fGCs with BHBA (n = 3). Bar graph showing the proportion of viable cells, early apoptotic cells and late apoptotic cells. For Fig. 1 C and E, we used one-way ANOVA for significant difference analysis (the different lowercases represent P < 0.05); for Fig. 1G, we used Student t-tests for significant difference analysis (*P < 0.05; **P < 0.01)

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