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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

Fig. 5

Effect of AN on the levels of proteins related to sonic hedgehog (SHH) signaling in porcine IVF embryos. Representative immunofluorescence images of A SHH, B PTCH1, C SMO, and D GLI1 in D2 embryos with and without AN treatment. Scale bar = 50 μm. E Relative fluorescence intensities of SHH, PTCH1, SMO, and GLI1 in D2 embryos with and without AN treatment (n = 30 per groups). F Relative expression levels of SHH signaling-related genes in D2 embryos with and without AN treatment (n = 3 per groups). Data are from three independent experiments, and different superscript letters indicate a significant difference (P < 0.05)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891