Fig. 4From: Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathwayEffect of AN on lipid metabolism in porcine IVF embryos. A Representative fluorescence images of lipid droplets in D2 embryos with and without AN treatment. Scale bar = 50 μm. B Relative fluorescence intensity of lipid droplets in D2 embryos with and without AN treatment (n = 24 per groups). C Representative fluorescence images of fatty acids in D2 embryos with and without AN treatment. Scale bar = 50 μm. D Relative fluorescence intensity of fatty acid staining in D2 embryos with and without AN treatment (n = 16 per groups). E Representative BODIPY-ATP fluorescence images in D2 embryos with and without AN treatment. Scale bar = 50 μm. F Relative ATP fluorescence intensity in D2 embryos with and without AN treatment (n = 24 per groups). Data are from three independent experiments, and different superscript letters indicate a significant difference (P < 0.05)Back to article page