Fig. 3

Effect of AN on mitochondrial function in porcine IVF embryos. A Representative fluorescence images of MitoTracker Deep Red staining of D2 embryos and D6 blastocysts with and without AN treatment. Scale bar = 100 μm. B Relative MitoTracker fluorescence intensity in D2 embryos and D6 blastocysts with and without AN treatment (D2; n = 51 per groups, D6; n = 25 per groups). C Representative TMRM fluorescence images in D2 embryos and D6 blastocysts with and without AN treatment. Scale bar = 50 μm. D Relative TMRM fluorescence intensity in D2 embryos and D6 blastocysts with and without AN treatment (D2; n = 23 per groups, D6; n = 20 per groups). Data are from three independent experiments, and different superscript letters indicate a significant difference (P < 0.05)