Fig. 2From: Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathwayEffect of AN on intracellular reactive oxygen species (ROS) and glutathione (GSH) levels in porcine IVF embryos. A Representative fluorescence images of CM-H2DCFDA staining in D2 embryos and D2 blastocysts with and without AN treatment. Scale bar = 100 μm. B Relative ROS fluorescence intensity in D2 embryos and D6 blastocysts with and without AN treatment (D2; n = 57 per groups, D6; n = 34 per groups). C Representative fluorescence images of CMF2HC staining of D2 embryos and D6 blastocysts with and without AN treatment. Scale bar = 100 μm. D Relative GSH fluorescence intensity in D2 embryos and D6 blastocysts with and without AN treatment (D2; n = 44 per groups, D6; n = 25 per groups). E Relative expression levels of antioxidation-related genes in D2 embryos and D6 blastocysts with and without AN treatment (n = 3 per groups). Data are from three independent experiments, and different superscript letters indicate a significant difference (P < 0.05)Back to article page