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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

Fig. 2

Effect of AN on intracellular reactive oxygen species (ROS) and glutathione (GSH) levels in porcine IVF embryos. A Representative fluorescence images of CM-H2DCFDA staining in D2 embryos and D2 blastocysts with and without AN treatment. Scale bar = 100 μm. B Relative ROS fluorescence intensity in D2 embryos and D6 blastocysts with and without AN treatment (D2; n = 57 per groups, D6; n = 34 per groups). C Representative fluorescence images of CMF2HC staining of D2 embryos and D6 blastocysts with and without AN treatment. Scale bar = 100 μm. D Relative GSH fluorescence intensity in D2 embryos and D6 blastocysts with and without AN treatment (D2; n = 44 per groups, D6; n = 25 per groups). E Relative expression levels of antioxidation-related genes in D2 embryos and D6 blastocysts with and without AN treatment (n = 3 per groups). Data are from three independent experiments, and different superscript letters indicate a significant difference (P < 0.05)

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