Fig. 8

The ERK inhibitor U0126 reverses adipogenesis inhibition induced by BIANCR knockdown A The protein expression levels of AKT, p-AKT, CTNNB1, p-CTNNB1, ERK1/2, p-ERK1/2, SMAD1/3/5, and p-SMAD1/3/5. B Quantitative of protein expression levels. C Oil Red O staining determined the amount of lipid droplets in intramuscular adipocytes (scale bar: 50 μm). D The mRNA expression levels of BIANCR and adipogenesis-related genes (PPARG, CEBPA, CEBPB, SREBF1, FABP4, and LPL). E The content of triacylglycerol in intramuscular adipocytes were evaluated utilizing triacylglycerol assay. F The protein expression levels of adipogenesis-related genes (PPARG and FABP4), ERK1/2 and p-ERK1/2. G Quantitative of protein expression levels. Results are presented as the means ± SD, *P < 0.05; ** P < 0.01; different lowercase letters indicate significant differences (P < 0.05)