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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: RNA-seq analysis reveals the critical role of the novel lncRNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway

Fig. 6

BIANCR promoted the proliferation and inhibited the apoptosis of intramuscular preadipocytes A EdU staining measured the number of intramuscular preadipocytes at proliferative stages. B Cell vitality was evaluated by CCK-8 assay. C The number of cells in G1(Gap 1 phase), S (Synthesis phase), and G2 (Gap 2 phase) were calculated by flow cytometry. D The mRNA expression levels of cell cycle related genes (CDK1, CDK2, CCND2, PCNA, p21, and p27). E The protein expression levels of cell cycle related genes (CDK1, PCNA, p21, and p27). F The number of viable non-apoptotic cells, viable apoptotic cells, non-viable apoptotic cells, and non-viable cells were counted by flow cytometry. G The mRNA expression levels of apoptotic related genes (BAD, CASP3, CASP6, BCL2 and BCL2L1). (H) The protein expression levels of apoptotic related genes (BAD, CASP3, and BCL2L1). Results are presented as the means ± SD, *P < 0.05; ** P < 0.01

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891