Effects of arginine replacement with L-citrulline on the arginine/nitric oxide metabolism in chickens: An animal model without urea cycle

Table 2 List of primers used for real-time PCR analysis

Target gene	Primer direction	Primer sequence (5′→3′)	Genebank accession No.
β-Actin	Forward	TGCGTGACATCAAGGAGAAG	NM_001145490.1
β-Actin	Reverse	TGCCAGGGTACATTGTGGTA
b^0,+AT	Forward	TGTGTTGCTCTCTAACTGGCTG	NM_001199133.1
b^0,+AT	Reverse	CCTCCTTTCTGTTGTCCTGTTC
EAAT3	Forward	ACCCTTTTGCCTTGGAAACT	XM_424930
EAAT3	Reverse	TTGAGATGTTTGCGTGAAG
PepT1	Forward	ACACGTTTGTTGCTCTGTGC	NM_204365
PepT1	Reverse	GACTGCCTGCCCAATTGTAT
Claudin1	Forward	ATGACCAGGTGAAGAAGATGC	NM_001013611.2
Claudin1	Reverse	TGCCCAGCCAATGAAGAG
Occludin	Forward	GGTTCCTCATCGTCATCCTGCTC	XM_025144248.1
Occludin	Reverse	GCCTCGTTCTTCACCCACTCCT
ZO-1	Forward	CTTCAGGTGTTTCTCTTCCTCCTC	XM_015278981.2
ZO-1	Reverse	CTGTGGTTTCATGGCTGGATC
pIgR	Forward	GGATCTGGAAGCCAGCAAT	ENSGALT00000001353
pIgR	Reverse	GAGCCAGAGCTTTGCTCAGA
MUC2	Forward	CCCTGGAAGTAGAGGTGACTG	XM_001234581.3
MUC2	Reverse	TGACAAGCCATTGAAGGACA
nNOS	Forward	CTCGGATGCACGGAAGTCCT	XM_004934480.1
nNOS	Reverse	CGTGAACCCAGCCCAAACAC
iNOS	Forward	GTGGTATGCTCTGCCTGCTGTTG	NM_204961.1
iNOS	Reverse	GTCTCGCACTCCAATCTCTGTTCC
eNOS	Forward	GGATGTGCTGCACGGTCTGC	JQ434761.1
eNOS	Reverse	AGGACGTGCTGCGGACACAG
ASS	Forward	GACACCTCCTGCATTCTGGT	NM_001013395
ASS	Reverse	CTTCTGGGCTGCATCAAAGT
ARG2	Forward	GCCAACTGTACGACTTTGGAG	AB159222
ARG2	Reverse	AGCTGTGTCCAGCAGCTACC
CPS1	Forward	GCCAACAGAGGACAGAACCA	NM_001045841
CPS1	Reverse	CAGGTGGGAGGGTAGAACTG
OTC	Forward	ACCTCCACTCCCTTGTCCTC	NM_204910
OTC	Reverse	TGTGCAGCTCCTTGTAATGC

b^0,+AT:b^0,+ AA transporter; EAAT3: Excitatory AA transporter 3; PepT1: Peptide transporter 1; ZO-1: Zona Occludens 1; MUC2: Mucin 2; pIgR: Polymeric Ig receptor; nNOS: Neuronal nitric oxide synthase; iNOS: Inducible nitric oxide synthase; eNOS: Endothelial nitric oxide synthase; ASS: Argininosuccinate synthetase; ARG2: Arginase 2; CPS1: Carbamoyl phosphate synthetase 1; OTC: Ornithine transcarbamylase

ISSN: 2049-1891