Table 1 Top 25 most abundant sperm-interacting proteins (SIPs) identified in the oviduct fluid (OF) derived from the ampulla and isthmus just before (Pre-ov) and after (Post-ov) ovulation
From: The sperm-interacting proteome in the bovine isthmus and ampulla during the periovulatory period
| Â | Â | Â | OF:control sperm ratio in | Â | Â | |||
|---|---|---|---|---|---|---|---|---|
Protein name | Gene symbol | Mean NWS in OF-treated sperm | Pre-ov isthmus | Pre-ov ampulla | Post-ov isthmus | Post-ov ampulla | Predicted secretion pathways | Reported role in sperm physiology and fertilization |
Myosin heavy chain 9 | MYH9 | 284.4 | 45.9 | 66.4 | 54.3 | 119.4 | EVs | OVGP1 partner on human sperm [26] |
Myosin heavy chain 14 | MYH14 | 184.0 | 66.4 | 75.3 | 86.2 | 140.7 | EVs | - |
AHNAK nucleoprotein | AHNAK | 101.4 | 512.7 | 341.1 | 509.2 | 287.5 | EVs | - |
Endoplasmic reticulum chaperone BiP | HSPA5 | 83.8 | 3.3 | 3.0 | 3.5 | 4.5 | EVs and classical | Human sperm capacitation [39] and binding to zona pellucida [40] |
Tubulin polymerization-promoting protein family member 3 | TPPP3 | 81.6 | 17.8 | 17.4 | 17.9 | 7.2 | EVs | - |
Myosin-10 | MYH10 | 70.3 | No detection in control | EVs | - | |||
Heat shock protein beta-1 | HSPB1 | 59.9 | 134.9 | 284.1 | 199.6 | 179.0 | EVs | - |
Myosin light polypeptide 6 | MYL6 | 58.1 | 22.3 | 31.5 | 33.9 | 34.0 | EVs and unconv | - |
Oviduct-specific glycoprotein | OVGP1 | 52.5 | 28.7 | 24.5 | 21.3 | 22.1 | EVs | Bovine sperm capacitation [41], binding to zona pellucida and monospermy [42] |
Heat shock cognate 71 kDa protein | HSPA8 | 38.6 | 3.0 | 3.3 | 2.7 | 2.5 | EVs | Bovine sperm survival, membrane fluidity and monospermy [21] |
Tropomodulin 3 | TMOD3 | 36.0 | 23.9 | 50.2 | 44.0 | 34.7 | Unconv | - |
Protein disulfide-isomerase | PDIA3 | 33.7 | 4.7 | 4.2 | 5.3 | 3.4 | EVs and classical | Human sperm binding to zona pellucida [43] |
Keratin, type II cytoskeletal 8 | KRT8 | 32.7 | 332.5 | 129.3 | 338.4 | 66.3 | EVs | - |
Actin, cytoplasmic 1 | ACTB | 31.7 | 2.9 | 4.7 | 3.9 | 4.1 | EVs | - |
Actin, cytoplasmic 2 | ACTG1 | 31.5 | 2.9 | 4.5 | 3.7 | 3.9 | EVs | - |
Endoplasmin | HSP90B1 | 28.9 | 4.2 | 4.1 | 3.4 | 5.3 | EVs and classical | KO mouse unable to fertilize oocytes [44] Binding partner of PDIA4 for monospermy in pigs [45] |
Hypoxia up-regulated 1 | HYOU1 | 27.5 | 5.6 | 3.2 | 5.3 | 4.7 | EVs | - |
Elongation factor 1-alpha 1 | EEF1A1 | 23.4 | 6.9 | 13.4 | 11.5 | 8.1 | EVs and unconv | - |
Tropomyosin alpha-3 chain | TPM3 | 23.3 | No detection in control | EVs and unconv | - | |||
Brain acid soluble protein 1 | BASP1 | 22.9 | 5.1 | 8.9 | 4.1 | 4.5 | EVs | - |
Annexin A1 | ANXA1 | 22.8 | 2.1 | 3.0 | 1.8 | 2.8 | EVs and unconv | Bovine sperm binding to oviduct epithelial cells [46] |
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | SLC9A3R1 | 21.6 | No detection in control | EVs | - | |||
Protein disulfide-isomerase A4 | PDIA4 | 20.7 | 26.8 | 26.8 | 27.3 | 31.7 | EVs and classical | Penetration rate and monospermy in pigs [47] Binding partner of HSP90B1 for monospermy in pigs [45] |
Annexin A2 | ANXA2 | 20.6 | 69.6 | 118.3 | 69.2 | 78.9 | EVs and unconv | Bovine sperm binding to oviduct epithelial cells [46] |
40S ribosomal protein S3a | RPS3A | 19.8 | 74.4 | 77.3 | 70.2 | 29.7 | EVs and unconv | - |