Fig. 1
Number, DNA fragmentation, and head size of the spermatozoa selected by thermotaxis. A Number of spermatozoa selected by thermotaxis with the medium supplemented with 5 mmol/L of caffeine in the thermotactic migration unit, thermotaxis (Tx) group (36 to 39 ℃), the inverted control (39 to 36 ℃) and the constant control temperatures (36 to 36 ℃ and 39 to 39 ℃). The asterisk indicates significant differences between groups (Kruskal-Wallis, P < 0.05). The initial number of spermatozoa loaded in the thermotaxis system was 10 × 106 spz/mL per separation unit, n = 6. B Percentage of DNA fragmentation in the spermatozoa selected by thermotaxis (Tx) and the not-migrated spermatozoa (NM), n = 8. Asterisk indicates significant differences between groups. C Area of the head size from spermatozoa. Number of spermatozoa migrated by thermotaxis (Tx) and not-migrated (NM) (4 repetitions; n = 400 spermatozoa analyzed for repetition). χ², P < 0.01. D Representation of the distribution of the head area value of spermatozoa migrated by thermotaxis (Tx) and not-migrated (NM)