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Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Isorhamnetin protects porcine oocytes from zearalenone-induced reproductive toxicity through the PI3K/Akt signaling pathway

Fig. 7

Isorhamnetin activated PI3K/Akt pathway in response to ZEA-induced meiosis arrest. Oocytes were co-cultured in ZEA (5 μmol/L), ISO (10 μmol/L) and LY294002 (20 μmol/L) for 44 h. A Western blot of PI3K, P-Akt and Akt protein in Control, ZEA and ZEA + ISO. B–C The ratios of PI3K to GAPDH and P-Akt to Akt expression were normalized, respectively. D Representative pictures of nuclear maturation in Control, ZEA, ZEA + ISO and ZEA + ISO + LY294002. E The percentage of polar extrusion was calculated. Various letters (P < 0.05) designate significant differences. Data are presented by average ± SEM

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891