Fig. 3

Isorhamnetin relieved oxidative stress caused by ZEA. Oocytes were treated with ZEA (5 μmol/L) for 44 h in the presence or absence of isorhamnetin (10 μmol/L). A Representative micrographs of cumulus cells expansion in Control, ZEA and ZEA + ISO. B The cumulus expansion area was calculated in Control, ZEA and ZEA + ISO. C Representative images of ROS fluorescence in Control, ZEA and ZEA + ISO. D The fluorescence intensity of ROS was measured. E Western blot of SOD2 protein in Control, ZEA and ZEA + ISO. F Ratios of SOD2 to GAPDH expression. Various letters (P < 0.05) designate significant differences. Data are presented by average ± SEM