Fig. 2

Isorhamnetin inhibited ZEA-caused apoptosis. Oocytes were cultured with ZEA (5 μmol/L) for 44 h with or without various concentrations of isorhamnetin (5, 10, 20 and 30 μmol/L). A Representative photomicrographs. (a) Early apoptotic oocyte. (b) Normal oocyte. B The ratios of early apoptosis was calculated at various groups. C Western blotting of Bcl-2, Bax and C-Casp3 were shown in different treatments. D–G The ratios of Bcl-2 to GAPDH, Bax to GAPDH, Bax to Bcl-2 and C-Caspase-3 to GAPDH expression were normalized, respectively. Various letters (P < 0.05) designate significant differences. Data are presented by average ± SEM