Table 2 Panel of porcine oligonucleotide primers used for real-time PCR
Target gene | Accession No. | Primers (5'→3') | Amplicon length, bp |
|---|---|---|---|
IL6 | NM_214399.1 | F: GACAAAGCCACCACCCCTAA | 69 |
R: CTCGTTCTGTGACTGCAGCTTATC | |||
CXCL8 | NM_213867.1 | F: TGCACTTACTCTTGCCAGAACTG | 82 |
R: CAAACTGGCTGTTGCCTTCTT | |||
IL10 | NM_214041.1 | F: GCCTTCGGCCCAGTGAA | 71 |
R: AGAGACCCGGTCAGCAACAA | |||
IL17A | NM_001005729.1 | F: CCCTGTCACTGCTGCTTCTG | 57 |
R: TCATGATTCCCGCCTTCAC | |||
IFNG | NM_213948.1 | F: TCTAACCTAAGAAAGCGGAAGAGAA | 81 |
R: TTGCAGGCAGGATGACAATTA | |||
TNFα | NM_214022.1 | F: TGGCCCCTTGAGCATCA | 68 |
R: CGGGCTTATCTGAGGTTTGAGA | |||
TGFB1 | NM_214015.1 | F: AGGGCTACCATGCCAATTTCT | 101 |
R: CGGGTTGTGCTGGTTGTACA | |||
TLR4 | NM_001293317.1 | F: TGCATGGAGCTGAATTTCTACAA | 140 |
R: GATAAATCCAGCACCTGCAGTTC | |||
MUC1 | XM_001926883.1 | F: ACACCCATGGGCGCTATGT | 68 |
R: GCCTGCAGAAACCTGCTCAT | |||
MUC2 | XM_021082584.1 | F: CAACGGCCTCTCCTTCTCTGT | 70 |
R: GCCACACTGGCCCTTTGT | |||
CLND1 | NM_001244539.1 | F: CTGGGAGGTGCCCTACTTTG | 72 |
R: TGGATAGGGCCTTGGTGTTG | |||
CLND3 | NM_001160075.1 | F: GAGGGCCTGTGGATGAACTG | 65 |
R: GAGTCGTACACTTTGCACTGCAT | |||
CCK | NM_214237.2 | F: GGACCCCAGCCACAGAATAA | 61 |
R: GCGCCGGCCAAAATC | |||
FABP2 | NM_001031780.1 | F: CAGCCTCGCAGACGGAACTGAA | 102 |
R: GTGTTCTGGGCTGTGCTCCAAGA | |||
SLC2A2/GLUT2 | NM_001097417.1 | F: CCAGGCCCCATCCCCTGGTT | 96 |
R:Â GCGGGTCCAGTTGCTGAATGC | |||
SLC2A5/GLUT5 | XM_021095282.1 | F: CCCAGGAGCCGGTCAAG | 60 |
R:Â TCAGCGTCGCCAAAGCA | |||
SLC5A1/SGLT1 | NM_001164021 | F: GGCTGGACGAAGTATGGTG | 153 |
R:Â ACAACCACCCAAATCAGAGC | |||
SLC15A1/PEPT1 | NM_214347.1 | F: GGATAGCCTGTACCCCAAGCT | 73 |
R: CATCCTCCACGTGCTTCTTGA | |||
NPY | NM_001256367.1 | F: CAGGCAGAGATACGGAAAACG | 71 |
R: TCCGTGCCTCTCTCATCAAG | |||
GLP1 | NM_001256594.1 | F: CAGTGCAGAAATGGCGAGAA | 61 |
R: GGTGGAGCCTCAGTCAGGAA | |||
PYY | XM_005668763.1 | F: CTCCTGATTCGGTTTGCAGAA | 61 |
R: GGACAGGAGCAGCAGGAAGA | |||
SELENOP | NM_001134823.1 | F:CAGGCCAGCTGATACCTGTGT | 83 |
R:TTAGAATATCCTTCTTTCTCCAGTTTTACTC | |||
DIO1 | NM_001001627.1 | F:GGCTCTGGGTGCTCTTTCAG | 69 |
R:CAGGAAACAATGTCATGAGCACTT | |||
TXNRD1 | NM_214154.3 | F:CACCGTGACGGACTCAAAACT | 72 |
R:GCTTGAGGCTGGTGACTTCAT | |||
H3F3A | NM_001014389.2 | F:CATGGCTCGTACAAAGCAGA | 136 |
R:ACCAGGCCTGTAACGATGAG | |||
ACTB | AY550069.1 | F:CAAATGCTTCTAGGCGGACTGT | 75 |
R:TCTCATTTTCTGCGCAAGTT | |||
YWZHAZ | XM_001927228.1 | F:GGACATCGGATACCCAAGGA | 71 |
R:AAGTTGGAAGGCCGGTTAATTT |